Cloning and localization of heparanase in bovine placenta

K. Kizaki, O. Yamada, H. Nakano, T. Takahashi, Nobuhiko Yamauchi, K. Imai, Kazuha Hashizume

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Heparanase (HPA) degrades heparan sulfate proteoglycan in the extracellular matrix. To understand its role during implantation and placental development in bovine placentae, we cloned and characterized a full-length cDNA encoding bovine HPA and identified HPA localization in placentae. A full-length bovine HPA cDNA was cloned with a 1635 nucleotide open-reading-frame corresponding to a protein of 545 amino acids. The predicted amino acid sequence shares 80.0% and 76.5% identity with human and rat HPA, respectively. In placentomes of 60 and 210 days' gestation, in situ hybridization demonstrated HPA mRNA expression in binucleate cells. Binucleate cells may be a source of HPA throughout gestation in bovine placentae; they may assume specific role(s) in foetal and maternal dialogue. Western blot analysis of bovine placental extracts (day 60) was performed using anti-bovine HPA antibody prepared by immunization of rabbits with synthetic peptide conjugate corresponding to amino acid residues 474-489 of bovine HPA; it showed two immunoreactive proteins with approximate molecular weights of 55 kDa and 65 kDa. Further, immunofluoresence double staining of HPA and placental lactogen (PL) revealed that binucleate cells expressing HPA had immunoreactivity of PL. These results suggest that HPA is specifically expressed in bovine placental binucleate cells and that it may take migratory roles in placentogenesis for degrading the extracellular matrix.

Original languageEnglish
Pages (from-to)424-430
Number of pages7
JournalPlacenta
Volume24
Issue number4
DOIs
Publication statusPublished - Jan 1 2003

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Placenta
Organism Cloning
Placental Lactogen
heparanase
Extracellular Matrix
Placental Extracts
Complementary DNA
Amino Acids
Placentation
Pregnancy
Heparan Sulfate Proteoglycans
Open Reading Frames
In Situ Hybridization
Amino Acid Sequence
Immunization
Proteins
Nucleotides
Molecular Weight
Western Blotting
Mothers

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

Cite this

Kizaki, K., Yamada, O., Nakano, H., Takahashi, T., Yamauchi, N., Imai, K., & Hashizume, K. (2003). Cloning and localization of heparanase in bovine placenta. Placenta, 24(4), 424-430. https://doi.org/10.1053/plac.2002.0909

Cloning and localization of heparanase in bovine placenta. / Kizaki, K.; Yamada, O.; Nakano, H.; Takahashi, T.; Yamauchi, Nobuhiko; Imai, K.; Hashizume, Kazuha.

In: Placenta, Vol. 24, No. 4, 01.01.2003, p. 424-430.

Research output: Contribution to journalArticle

Kizaki, K, Yamada, O, Nakano, H, Takahashi, T, Yamauchi, N, Imai, K & Hashizume, K 2003, 'Cloning and localization of heparanase in bovine placenta', Placenta, vol. 24, no. 4, pp. 424-430. https://doi.org/10.1053/plac.2002.0909
Kizaki K, Yamada O, Nakano H, Takahashi T, Yamauchi N, Imai K et al. Cloning and localization of heparanase in bovine placenta. Placenta. 2003 Jan 1;24(4):424-430. https://doi.org/10.1053/plac.2002.0909
Kizaki, K. ; Yamada, O. ; Nakano, H. ; Takahashi, T. ; Yamauchi, Nobuhiko ; Imai, K. ; Hashizume, Kazuha. / Cloning and localization of heparanase in bovine placenta. In: Placenta. 2003 ; Vol. 24, No. 4. pp. 424-430.
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