Cloning and sequencing of cdna coding for cry j i, a major allergen of Japanese cedar pollen

Toshio Sone, Naoki Komiyama, Kimiko Shimizu, Takahiro Kusakabe, Keiko Morikubo, Kohsuke Kino

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

cDNA clones coding for Cry j I, a major allergen of the Japanese cedar (Cryptomeria japonica) pollen, have been isolated. Two of the clones were sequenced and found to code for a putative 21-residue signal peptide and a 353-residue mature protein with a derived molecular weight of 38.5 KDa. Five possible N-linked glycosylation sites were found in the sequence. Comparison of the nucleotide sequences of the two clones revealed sixteen nucleotide differences and these led to five amino acid exchanges in the mature allergen, indicating that an isoform of the Cry j I molecule exists. The deduced amino acid sequence of the Cry j I shows 46-48% identities with those of the Amb a I family and Amb a II, the major allergens of short ragweed. These findings should facilitate study of the structure-function relationship between the allergen and the immunocompetent cells.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume199
Issue number2
DOIs
Publication statusPublished - Mar 15 1994

Fingerprint

Cryptomeria
Cloning
Pollen
Allergens
Organism Cloning
Clone Cells
Nucleotides
Glycosylation
Ambrosia
Amino Acids
Protein Sorting Signals
Amino Acid Sequence
Protein Isoforms
Complementary DNA
Molecular Weight
Molecular weight
Molecules
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and sequencing of cdna coding for cry j i, a major allergen of Japanese cedar pollen. / Sone, Toshio; Komiyama, Naoki; Shimizu, Kimiko; Kusakabe, Takahiro; Morikubo, Keiko; Kino, Kohsuke.

In: Biochemical and Biophysical Research Communications, Vol. 199, No. 2, 15.03.1994, p. 619-625.

Research output: Contribution to journalArticle

Sone, Toshio ; Komiyama, Naoki ; Shimizu, Kimiko ; Kusakabe, Takahiro ; Morikubo, Keiko ; Kino, Kohsuke. / Cloning and sequencing of cdna coding for cry j i, a major allergen of Japanese cedar pollen. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 199, No. 2. pp. 619-625.
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