TY - JOUR
T1 - Cloning and sequencing of the hup gene encoding the histone-like protein HS1 of Streptomyces lividans
AU - Yokoyama, Eiji
AU - Doi, Katsumi
AU - Ogata, Seiya
N1 - Funding Information:
This work was supported in part by the Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Science, Sports and Culture of Japan (08456055) and the Waksman Foundation of Japan Inc. We are grateful to D.A. Hopwood, K.F. Chater and T. Kieser (John Innes Centre, Norwich, UK) for providing S. coelicolor M145, S. lividans TK24 and S. lividans ZX7, and to M. Ohara for critical readings of the manuscript.
PY - 1997/8/7
Y1 - 1997/8/7
N2 - The hup gene encoding the histone-like HU-type protein HSI of Streptomyces lividans TK24 was cloned and sequenced. The deduced N-terminal amino acid sequence, molecular mass (9851 Da) and pI (9.95) are in good agreement with characteristics of the HS1 protein. The hup transcript of about 500 nucleotides was detected. The 2.3-kb HincII fragment containing the hup gene hybridized with the AseI fragment C in the 9-10 o'clock region of the chromosome of S. lividans ZX7.
AB - The hup gene encoding the histone-like HU-type protein HSI of Streptomyces lividans TK24 was cloned and sequenced. The deduced N-terminal amino acid sequence, molecular mass (9851 Da) and pI (9.95) are in good agreement with characteristics of the HS1 protein. The hup transcript of about 500 nucleotides was detected. The 2.3-kb HincII fragment containing the hup gene hybridized with the AseI fragment C in the 9-10 o'clock region of the chromosome of S. lividans ZX7.
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U2 - 10.1016/S0167-4781(97)00089-4
DO - 10.1016/S0167-4781(97)00089-4
M3 - Article
C2 - 9294002
AN - SCOPUS:0030852930
VL - 1353
SP - 103
EP - 106
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -