Cloning and Structure of the Human Adrenodoxin Gene

Chi Yao Chang, du an wu, Char Chang Lai, Walter L. Miller, Bon Chu Chung

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Adrenodoxin is an iron–sulfur protein that serves as an electron transport intermediate for all mitochondrial forms of cytochrome P450. To facilitate studying the regulation of adrenodoxin, we have cloned and determined the structure of the human adrenodoxin gene. It spans more than 20 kb, containing four exons and three introns. The first exon encodes the 60-amino-acid signal peptide, directing transport of the protein into the inner mitochondrial matrix. The mature peptide of 124 amino acids is encoded by the other three exons. The third exon encodes the portion of the protein containing the iron–sulfur center and a domain which binds other components of the electron transport chain. The transcriptional start sites were determined by primer extension and S1 nuclease mapping. The 5′-flanking region of this gene contains canonical promoters including a TATA box at nucleotide position −30 and two GC boxes at nucleotide positions −60 and −100. The sequence at nucleotides −234 to −252 is also highly homologous to the glucocorticoid-responsive element and the estrogen-responsive element.

Original languageEnglish
Pages (from-to)609-615
Number of pages7
JournalDNA
Volume7
Issue number9
DOIs
Publication statusPublished - Jan 1 1988

Fingerprint

Adrenodoxin
Cloning
Organism Cloning
Exons
Genes
Nucleotides
Electron Transport
Amino Acids
TATA Box
5' Flanking Region
Protein Sorting Signals
Introns
Cytochrome P-450 Enzyme System
Glucocorticoids
Carrier Proteins
Estrogens
Proteins
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Chang, C. Y., wu, D. A., Lai, C. C., Miller, W. L., & Chung, B. C. (1988). Cloning and Structure of the Human Adrenodoxin Gene. DNA, 7(9), 609-615. https://doi.org/10.1089/dna.1988.7.609

Cloning and Structure of the Human Adrenodoxin Gene. / Chang, Chi Yao; wu, du an; Lai, Char Chang; Miller, Walter L.; Chung, Bon Chu.

In: DNA, Vol. 7, No. 9, 01.01.1988, p. 609-615.

Research output: Contribution to journalArticle

Chang, CY, wu, DA, Lai, CC, Miller, WL & Chung, BC 1988, 'Cloning and Structure of the Human Adrenodoxin Gene', DNA, vol. 7, no. 9, pp. 609-615. https://doi.org/10.1089/dna.1988.7.609
Chang CY, wu DA, Lai CC, Miller WL, Chung BC. Cloning and Structure of the Human Adrenodoxin Gene. DNA. 1988 Jan 1;7(9):609-615. https://doi.org/10.1089/dna.1988.7.609
Chang, Chi Yao ; wu, du an ; Lai, Char Chang ; Miller, Walter L. ; Chung, Bon Chu. / Cloning and Structure of the Human Adrenodoxin Gene. In: DNA. 1988 ; Vol. 7, No. 9. pp. 609-615.
@article{a0b6749b73f44e1ab80dac6483973b6f,
title = "Cloning and Structure of the Human Adrenodoxin Gene",
abstract = "Adrenodoxin is an iron–sulfur protein that serves as an electron transport intermediate for all mitochondrial forms of cytochrome P450. To facilitate studying the regulation of adrenodoxin, we have cloned and determined the structure of the human adrenodoxin gene. It spans more than 20 kb, containing four exons and three introns. The first exon encodes the 60-amino-acid signal peptide, directing transport of the protein into the inner mitochondrial matrix. The mature peptide of 124 amino acids is encoded by the other three exons. The third exon encodes the portion of the protein containing the iron–sulfur center and a domain which binds other components of the electron transport chain. The transcriptional start sites were determined by primer extension and S1 nuclease mapping. The 5′-flanking region of this gene contains canonical promoters including a TATA box at nucleotide position −30 and two GC boxes at nucleotide positions −60 and −100. The sequence at nucleotides −234 to −252 is also highly homologous to the glucocorticoid-responsive element and the estrogen-responsive element.",
author = "Chang, {Chi Yao} and wu, {du an} and Lai, {Char Chang} and Miller, {Walter L.} and Chung, {Bon Chu}",
year = "1988",
month = "1",
day = "1",
doi = "10.1089/dna.1988.7.609",
language = "English",
volume = "7",
pages = "609--615",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "9",

}

TY - JOUR

T1 - Cloning and Structure of the Human Adrenodoxin Gene

AU - Chang, Chi Yao

AU - wu, du an

AU - Lai, Char Chang

AU - Miller, Walter L.

AU - Chung, Bon Chu

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Adrenodoxin is an iron–sulfur protein that serves as an electron transport intermediate for all mitochondrial forms of cytochrome P450. To facilitate studying the regulation of adrenodoxin, we have cloned and determined the structure of the human adrenodoxin gene. It spans more than 20 kb, containing four exons and three introns. The first exon encodes the 60-amino-acid signal peptide, directing transport of the protein into the inner mitochondrial matrix. The mature peptide of 124 amino acids is encoded by the other three exons. The third exon encodes the portion of the protein containing the iron–sulfur center and a domain which binds other components of the electron transport chain. The transcriptional start sites were determined by primer extension and S1 nuclease mapping. The 5′-flanking region of this gene contains canonical promoters including a TATA box at nucleotide position −30 and two GC boxes at nucleotide positions −60 and −100. The sequence at nucleotides −234 to −252 is also highly homologous to the glucocorticoid-responsive element and the estrogen-responsive element.

AB - Adrenodoxin is an iron–sulfur protein that serves as an electron transport intermediate for all mitochondrial forms of cytochrome P450. To facilitate studying the regulation of adrenodoxin, we have cloned and determined the structure of the human adrenodoxin gene. It spans more than 20 kb, containing four exons and three introns. The first exon encodes the 60-amino-acid signal peptide, directing transport of the protein into the inner mitochondrial matrix. The mature peptide of 124 amino acids is encoded by the other three exons. The third exon encodes the portion of the protein containing the iron–sulfur center and a domain which binds other components of the electron transport chain. The transcriptional start sites were determined by primer extension and S1 nuclease mapping. The 5′-flanking region of this gene contains canonical promoters including a TATA box at nucleotide position −30 and two GC boxes at nucleotide positions −60 and −100. The sequence at nucleotides −234 to −252 is also highly homologous to the glucocorticoid-responsive element and the estrogen-responsive element.

UR - http://www.scopus.com/inward/record.url?scp=0023698129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023698129&partnerID=8YFLogxK

U2 - 10.1089/dna.1988.7.609

DO - 10.1089/dna.1988.7.609

M3 - Article

VL - 7

SP - 609

EP - 615

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 9

ER -