Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig

Ken Karasawa, Osamu Kuge, Kiyoshi Kawasaki, Masahiro Nishijima, Yasuko Nakano, Motowo Tomita, Kazuaki Yokoyama, Morio Setaka, Shoshichi Nojima

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In a previous study, we purified PAF-acetylhydrolase which converts PAF to an inactive metabolite, lysoPAF, from peritoneal fluid of guinea pigs subjected to experimental endotoxin shock and found that this purified enzyme had similar biochemical properties to the plasma enzyme. In this study, we isolated a homogeneous enzyme preparation from guinea pig plasma using a similar procedure. The molecular mass of this purified enzyme, as determined by SDS-PAGE was 58-63 kDa, larger than that (43 kDa) of the human enzyme. To elucidate the molecular structure of this enzyme and clarify its relationships with PAF-acetylhydrolases of other species, we isolated and sequenced a cDNA encoding this enzyme. Its cDNA contains an open reading frame encoding 436 amino acids and its predicted molecular mass (49 kDa) is lower than that of the native enzyme, suggesting that guinea pig plasma PAF-acetylhydrolase, unlike the human enzyme, is modified post-translationally, perhaps by glycosylation.

Original languageEnglish
Pages (from-to)838-844
Number of pages7
JournalJournal of biochemistry
Volume120
Issue number4
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Cloning
Platelet Activating Factor
Organism Cloning
Guinea Pigs
Plasmas
Enzymes
1-Alkyl-2-acetylglycerophosphocholine Esterase
Molecular mass
Complementary DNA
Glycosylation
Ascitic Fluid
Metabolites
Molecular Structure
Endotoxins
Molecular structure
Open Reading Frames
Polyacrylamide Gel Electrophoresis
Shock
Amino Acids
Fluids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. / Karasawa, Ken; Kuge, Osamu; Kawasaki, Kiyoshi; Nishijima, Masahiro; Nakano, Yasuko; Tomita, Motowo; Yokoyama, Kazuaki; Setaka, Morio; Nojima, Shoshichi.

In: Journal of biochemistry, Vol. 120, No. 4, 01.01.1996, p. 838-844.

Research output: Contribution to journalArticle

Karasawa, K, Kuge, O, Kawasaki, K, Nishijima, M, Nakano, Y, Tomita, M, Yokoyama, K, Setaka, M & Nojima, S 1996, 'Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig', Journal of biochemistry, vol. 120, no. 4, pp. 838-844. https://doi.org/10.1093/oxfordjournals.jbchem.a021488
Karasawa, Ken ; Kuge, Osamu ; Kawasaki, Kiyoshi ; Nishijima, Masahiro ; Nakano, Yasuko ; Tomita, Motowo ; Yokoyama, Kazuaki ; Setaka, Morio ; Nojima, Shoshichi. / Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. In: Journal of biochemistry. 1996 ; Vol. 120, No. 4. pp. 838-844.
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