Cloning, expression, and nucleotide sequence of the N-acyl-d-aspartate amidohydrolase gene from Alcaligenes xylosoxydans subsp. xylosoxydans A-6

Mamoru Wakayama, Eiki Watanabe, Yasuhiro Takenaka, Yoshiro Miyamoto, Yuko Tau, Kenji Sakai, Mitsuaki Moriguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The gene (termed daa) encoding N-acyl-d-aspartate (d-Asp) amidohydrolase (d-AAase) from the Alcaligenes xylosoxydans subsp. xylosoxydans (Alcaligenes A-6) was cloned in Escherichia coli (E. coli) JM109. The daa gene consists of 1,494 nucleotides and encodes 498 amino acid residues. The molecular weight of d-AAase was calculated to be 53,581. The N-terminal amino acid sequence (NH2-TDRSTLDDAP-) predicted by the nucleotide sequence matched exactly those of the purified d-AAase from both Alcaligenes A-6 and cloned E. coli, with the exception of the removal of the N-terminal methionine processed after translation. A comparison of the amino acid sequence of d-AAase with that of d-aminoacylase from Alcaligenes A-6 showed high overall homology (56%). d-AAase from Alcaligenes A-6 showed 25∼29% homology with Bacillus stearothermophilus, porcine, and human l-aminoacylases. The daa was highly expressed in E. coli, and the recombinant enzyme was purified to homogeneity with 17.8% yield.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Fermentation and Bioengineering
Volume80
Issue number4
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Amidohydrolases
Alcaligenes
Cloning
Nucleotides
Aspartic Acid
Organism Cloning
Genes
Escherichia coli
Amino acids
Amino Acids
Amino Acid Sequence
Geobacillus stearothermophilus
Bacilli
Methionine
Swine
Enzymes
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Cloning, expression, and nucleotide sequence of the N-acyl-d-aspartate amidohydrolase gene from Alcaligenes xylosoxydans subsp. xylosoxydans A-6. / Wakayama, Mamoru; Watanabe, Eiki; Takenaka, Yasuhiro; Miyamoto, Yoshiro; Tau, Yuko; Sakai, Kenji; Moriguchi, Mitsuaki.

In: Journal of Fermentation and Bioengineering, Vol. 80, No. 4, 01.01.1995, p. 311-317.

Research output: Contribution to journalArticle

Wakayama, Mamoru ; Watanabe, Eiki ; Takenaka, Yasuhiro ; Miyamoto, Yoshiro ; Tau, Yuko ; Sakai, Kenji ; Moriguchi, Mitsuaki. / Cloning, expression, and nucleotide sequence of the N-acyl-d-aspartate amidohydrolase gene from Alcaligenes xylosoxydans subsp. xylosoxydans A-6. In: Journal of Fermentation and Bioengineering. 1995 ; Vol. 80, No. 4. pp. 311-317.
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AU - Watanabe, Eiki

AU - Takenaka, Yasuhiro

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AU - Tau, Yuko

AU - Sakai, Kenji

AU - Moriguchi, Mitsuaki

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