Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon (Oncorhynchus rhodurus)

Yuichi Oba, Toshiaki Hirai, Yasutoshi Yoshiura, Michiyasu Yoshikuni, Hiroshi Kawauchi, Yoshitaka Nagahama

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

A gonadotropin receptor was cloned from amago salmon (Oncorhynchus rhodurus) ovarian follicles. This receptor (sGTH-R) belongs to the glycoprotein hormone receptor family with a large extracellular and seven-transmembrane domains. Its sequence homology is highest with mammalian LH receptors. Phylogenetic analysis reveals that sGTH-R is grouped with mammalian and chicken FSH and LH receptors, but not with mammalian TSH receptors. sGTH-R is expressed dominantly in the ovary and testis. Functional characterization examined with transiently transfected mammalian cells revealed increased intracellular cAMP level when exposed to mammalian and fish gonadotropins; the most potent hormone was salmon GTH II. These results indicate that the cloned cDNA encodes a functional amago salmon GTH receptor protein.

Original languageEnglish
Pages (from-to)584-590
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number2
DOIs
Publication statusPublished - Sep 24 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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