Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon (Oncorhynchus rhodurus)

Yuichi Oba, Toshiaki Hirai, Yasutoshi Yoshiura, Michiyasu Yoshikuni, Hiroshi Kawauchi, Yoshitaka Nagahama

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

A gonadotropin receptor was cloned from amago salmon (Oncorhynchus rhodurus) ovarian follicles. This receptor (sGTH-R) belongs to the glycoprotein hormone receptor family with a large extracellular and seven-transmembrane domains. Its sequence homology is highest with mammalian LH receptors. Phylogenetic analysis reveals that sGTH-R is grouped with mammalian and chicken FSH and LH receptors, but not with mammalian TSH receptors. sGTH-R is expressed dominantly in the ovary and testis. Functional characterization examined with transiently transfected mammalian cells revealed increased intracellular cAMP level when exposed to mammalian and fish gonadotropins; the most potent hormone was salmon GTH II. These results indicate that the cloned cDNA encodes a functional amago salmon GTH receptor protein.

Original languageEnglish
Pages (from-to)584-590
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number2
DOIs
Publication statusPublished - Sep 24 1999
Externally publishedYes

Fingerprint

Oncorhynchus
Gonadotropin Receptors
LH Receptors
Salmon
Cloning
Testis
Organism Cloning
Ovary
Complementary DNA
Hormones
FSH Receptors
Thyrotropin Receptors
Gonadotropins
Fish
Glycoproteins
Ovarian Follicle
Cells
Sequence Homology
Chickens
Fishes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon (Oncorhynchus rhodurus). / Oba, Yuichi; Hirai, Toshiaki; Yoshiura, Yasutoshi; Yoshikuni, Michiyasu; Kawauchi, Hiroshi; Nagahama, Yoshitaka.

In: Biochemical and Biophysical Research Communications, Vol. 263, No. 2, 24.09.1999, p. 584-590.

Research output: Contribution to journalArticle

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