Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family

Tohru Kiyono, Masatoshi Fujita, Yasuyuki Hayashi, Masahide Ishibashi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1307
Issue number1
DOIs
Publication statusPublished - Jun 3 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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