TY - JOUR
T1 - Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family
AU - Kiyono, Tohru
AU - Fujita, Masatoshi
AU - Hayashi, Yasuyuki
AU - Ishibashi, Masahide
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan.
PY - 1996/6/3
Y1 - 1996/6/3
N2 - A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.
AB - A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.
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U2 - 10.1016/0167-4781(96)00057-7
DO - 10.1016/0167-4781(96)00057-7
M3 - Article
C2 - 8652665
AN - SCOPUS:0029944252
SN - 0167-4781
VL - 1307
SP - 31
EP - 34
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1
ER -