Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase

Tatsurou Hamada, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast ω-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those deduced from the previously described genes encoding the ω-3 FA desaturases of A. thaliana. The absence of an N-terminal extension transit peptide in the deduced aa sequence of the cDNA clone and the accumulation pattern of the mRNA corresponding to this cDNA in leaf and root tissues indicate that the isolated cDNA encodes a tobacco microsomal co-3 FA desaturase.

Original languageEnglish
Pages (from-to)293-294
Number of pages2
JournalGene
Volume147
Issue number2
DOIs
Publication statusPublished - Sep 30 1994

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Fatty Acid Desaturases
Tobacco
Organism Cloning
Complementary DNA
Arabidopsis
Amino Acid Sequence
Chloroplasts
Gene Library
Genes
Clone Cells
Messenger RNA
Peptides

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase. / Hamada, Tatsurou; Kodama, Hiroaki; Nishimura, Mitsuo; Iba, Koh.

In: Gene, Vol. 147, No. 2, 30.09.1994, p. 293-294.

Research output: Contribution to journalArticle

Hamada, Tatsurou ; Kodama, Hiroaki ; Nishimura, Mitsuo ; Iba, Koh. / Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase. In: Gene. 1994 ; Vol. 147, No. 2. pp. 293-294.
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