Abstract
A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast ω-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those deduced from the previously described genes encoding the ω-3 FA desaturases of A. thaliana. The absence of an N-terminal extension transit peptide in the deduced aa sequence of the cDNA clone and the accumulation pattern of the mRNA corresponding to this cDNA in leaf and root tissues indicate that the isolated cDNA encodes a tobacco microsomal co-3 FA desaturase.
| Original language | English |
|---|---|
| Pages (from-to) | 293-294 |
| Number of pages | 2 |
| Journal | Gene |
| Volume | 147 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 30 1994 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Genetics
Cite this
Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase. / Hamada, Tatsurou; Kodama, Hiroaki; Nishimura, Mitsuo; Iba, Koh.
In: Gene, Vol. 147, No. 2, 30.09.1994, p. 293-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase
AU - Hamada, Tatsurou
AU - Kodama, Hiroaki
AU - Nishimura, Mitsuo
AU - Iba, Koh
PY - 1994/9/30
Y1 - 1994/9/30
N2 - A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast ω-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those deduced from the previously described genes encoding the ω-3 FA desaturases of A. thaliana. The absence of an N-terminal extension transit peptide in the deduced aa sequence of the cDNA clone and the accumulation pattern of the mRNA corresponding to this cDNA in leaf and root tissues indicate that the isolated cDNA encodes a tobacco microsomal co-3 FA desaturase.
AB - A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast ω-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those deduced from the previously described genes encoding the ω-3 FA desaturases of A. thaliana. The absence of an N-terminal extension transit peptide in the deduced aa sequence of the cDNA clone and the accumulation pattern of the mRNA corresponding to this cDNA in leaf and root tissues indicate that the isolated cDNA encodes a tobacco microsomal co-3 FA desaturase.
UR - http://www.scopus.com/inward/record.url?scp=0028086460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028086460&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(94)90084-1
DO - 10.1016/0378-1119(94)90084-1
M3 - Article
C2 - 7926817
AN - SCOPUS:0028086460
VL - 147
SP - 293
EP - 294
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -