Cloning of rat p47phox and comparison with human p47 phox

Michiharu Tanabe, Olof Rådmark, Takashi Watanabe, Akira Shiose, Hideki Sumimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 1 2005

Fingerprint

Cloning
Rats
Organism Cloning
Complementary DNA
Arachidonate 12-Lipoxygenase
Two-Hybrid System Techniques
src Homology Domains
Cell-Free System
NADPH Oxidase
Phagocytes
4-ethoxymethylene-2-phenyl-2-oxazoline-5-one
Glutathione Transferase
Hybrid systems
Gene Library
Proline
Yeast
Open Reading Frames
Amplification
Oxidoreductases
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

Cite this

Cloning of rat p47phox and comparison with human p47 phox. / Tanabe, Michiharu; Rådmark, Olof; Watanabe, Takashi; Shiose, Akira; Sumimoto, Hideki.

In: DNA Sequence - Journal of DNA Sequencing and Mapping, Vol. 16, No. 1, 01.02.2005, p. 65-68.

Research output: Contribution to journalArticle

Tanabe, Michiharu ; Rådmark, Olof ; Watanabe, Takashi ; Shiose, Akira ; Sumimoto, Hideki. / Cloning of rat p47phox and comparison with human p47 phox. In: DNA Sequence - Journal of DNA Sequencing and Mapping. 2005 ; Vol. 16, No. 1. pp. 65-68.
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