Cloning of rat p47phox and comparison with human p47 phox

Michiharu Tanabe, Olof Rådmark, Takashi Watanabe, Akira Shiose, Hideki Sumimoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

Fingerprint Dive into the research topics of 'Cloning of rat p47<sup>phox</sup> and comparison with human p47 <sup>phox</sup>'. Together they form a unique fingerprint.

Cite this