TY - JOUR
T1 - Cloning of rat p47phox and comparison with human p47 phox
AU - Tanabe, Michiharu
AU - Rådmark, Olof
AU - Watanabe, Takashi
AU - Shiose, Akira
AU - Sumimoto, Hideki
N1 - Funding Information:
This study was supported by grants from the Scandinavia-Japan Sasakawa Foundation (No.00-16), the Swedish Research Council (03X-217), and EU (QLG1-CT-2001-01521). We thank Drs Patrick Provost, Hideki Kamitani and Hisayo Okamoto for excellent technical assistance and discussions.
PY - 2005/2
Y1 - 2005/2
N2 - A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.
AB - A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.
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U2 - 10.1080/10425170400028228
DO - 10.1080/10425170400028228
M3 - Article
C2 - 16040349
AN - SCOPUS:24344501343
SN - 1042-5179
VL - 16
SP - 65
EP - 68
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
IS - 1
ER -