Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage

O. Maruyama, H. Nishimori, T. Katagiri, Y. Miki, A. Ueno, Yusuke Nakamura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have isolated a novel human gene that is expressed specifically in primary spermatocytes in the testis. The cDNA contains an open reading frame of 1356 bp, encoding a 452-amino-acid protein that includes a basic Helix-Loop-Helix (bHLH) motif. The gene, which was mapped to chromosome region 20q13.3 → qter by fluorescence in situ hybridization, consists of six exons and spans approximately 24 kb of genomic DNA. Immunohistochemical staining located the gene product exclusively in cell nuclei of primary spermatocytes at the pachytene stage, but not in those at the leptonema stage. We named this gene TCFL5 (transcription factor-like 5, basic helix-loop-helix). The cell-type and stage-specific expression of TCFL5 indicates that this protein may function in a crucial role in spermatogenesis as a transcription factor by regulating cell proliferation or differentiation of cells through binding to a specific DNA sequence like other bHLH molecules.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalCytogenetics and Cell Genetics
Volume82
Issue number1-2
Publication statusPublished - Oct 27 1998
Externally publishedYes

Fingerprint

Helix-Loop-Helix Motifs
Pachytene Stage
Spermatocytes
Organism Cloning
Genes
Proteins
Transcription Factors
Spermatogenesis
Cell Nucleus
Fluorescence In Situ Hybridization
Open Reading Frames
Testis
Cell Differentiation
Exons
Complementary DNA
Chromosomes
Cell Proliferation
Staining and Labeling
Amino Acids
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage. / Maruyama, O.; Nishimori, H.; Katagiri, T.; Miki, Y.; Ueno, A.; Nakamura, Yusuke.

In: Cytogenetics and Cell Genetics, Vol. 82, No. 1-2, 27.10.1998, p. 41-45.

Research output: Contribution to journalArticle

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AU - Nakamura, Yusuke

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