Cloning of xenopus Dr1 (TBP-Binding repressor) and its expression in oocytes and early embryos

Michiyo Nagano, Chie Koga, Kosuke Tashiro, Fumihiko Kugawa, Koichiro Shiokawa

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Abstract

With a final goal to study how TBP-binding repressor Dr1 regulates transcription in Xenopus early embryos, we cloned its cDNA from Xenopus liver cDNA library. The cDNA was 1,986 bp long, with the open reading frame coding for 175 amino acids, whose sequence was highly homologous to its human counterpart. Xenopus Dr1 mRNA was expressed from the earliest stage of oogenesis, inherited as maternal mRNA at a high level, but its level became low at and after the neurula stage where RNA synthetic activity is strongly activated. Dr1 mRNA occurred in larger amounts in the animal half than in the vegetal half in 8-cell stage embryos, and in neurula and tailbud stage embryos its distribution was slightly larger in the anterior part than in the posterior part. These data show that Dr1 mRNA is expressed in a temporally and spatially regulated manner, and its occurrence at higher levels in earlier stage embryos may be responsible for their low transcriptional activity.

Original languageEnglish
Pages (from-to)561-565
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number3
DOIs
Publication statusPublished - Feb 24 1997
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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