Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc

Wayne Wei Ku Lai, Pei Hung Hsiao, Yann Guiguen, Bon Chu Chung

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

P450scc and 3β-HSD cDNA were isolated from a zebrafish λgt10 cDNA library using trout SCC and 3β-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3β-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3β- HSD and 53% similarities with the mouse 3β-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3β-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.

Original languageEnglish
Pages (from-to)927-931
Number of pages5
JournalEndocrine Research
Volume24
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

3-Hydroxysteroid Dehydrogenases
Zebrafish
Trout
Organism Cloning
Complementary DNA
Amino Acids
Genetic Models
Alternative Splicing
Gene Library
Proteins
Steroids

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc. / Lai, Wayne Wei Ku; Hsiao, Pei Hung; Guiguen, Yann; Chung, Bon Chu.

In: Endocrine Research, Vol. 24, No. 3-4, 01.01.1998, p. 927-931.

Research output: Contribution to journalArticle

Lai, Wayne Wei Ku ; Hsiao, Pei Hung ; Guiguen, Yann ; Chung, Bon Chu. / Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc. In: Endocrine Research. 1998 ; Vol. 24, No. 3-4. pp. 927-931.
@article{e541ce0b4aa142b4aba4031b4be0428f,
title = "Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc",
abstract = "P450scc and 3β-HSD cDNA were isolated from a zebrafish λgt10 cDNA library using trout SCC and 3β-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78{\%} similarities with the trout SCC and 58{\%} with the human SCC. As for 3β-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77{\%} amino acid similarities with trout 3β- HSD and 53{\%} similarities with the mouse 3β-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3β-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.",
author = "Lai, {Wayne Wei Ku} and Hsiao, {Pei Hung} and Yann Guiguen and Chung, {Bon Chu}",
year = "1998",
month = "1",
day = "1",
doi = "10.3109/07435809809032708",
language = "English",
volume = "24",
pages = "927--931",
journal = "Endocrine Research",
issn = "0743-5800",
publisher = "Informa Healthcare",
number = "3-4",

}

TY - JOUR

T1 - Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc

AU - Lai, Wayne Wei Ku

AU - Hsiao, Pei Hung

AU - Guiguen, Yann

AU - Chung, Bon Chu

PY - 1998/1/1

Y1 - 1998/1/1

N2 - P450scc and 3β-HSD cDNA were isolated from a zebrafish λgt10 cDNA library using trout SCC and 3β-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3β-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3β- HSD and 53% similarities with the mouse 3β-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3β-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.

AB - P450scc and 3β-HSD cDNA were isolated from a zebrafish λgt10 cDNA library using trout SCC and 3β-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3β-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3β- HSD and 53% similarities with the mouse 3β-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3β-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.

UR - http://www.scopus.com/inward/record.url?scp=0032440945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032440945&partnerID=8YFLogxK

U2 - 10.3109/07435809809032708

DO - 10.3109/07435809809032708

M3 - Article

VL - 24

SP - 927

EP - 931

JO - Endocrine Research

JF - Endocrine Research

SN - 0743-5800

IS - 3-4

ER -