Cloning of zebrafish cDNA for 3β-hydroxysteroid dehydrogenase and P450scc

Wayne Wei Ku Lai, Pei Hung Hsiao, Yann Guiguen, Bon Chu Chung

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

P450scc and 3β-HSD cDNA were isolated from a zebrafish λgt10 cDNA library using trout SCC and 3β-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3β-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3β- HSD and 53% similarities with the mouse 3β-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3β-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.

Original languageEnglish
Pages (from-to)927-931
Number of pages5
JournalEndocrine Research
Volume24
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Endocrinology

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