Clusterin/apolipoprotein J is associated with cortical Lewy bodies: Immunohistochemical study in cases with α-synucleinopathies

Kensuke Sasaki, Katsumi Doh-ura, Yoshinobu Wakisaka, Toru Iwaki

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Clusterin/apolipoprotein J protein expression in cases with "α-synucleinopathies", such as Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA), was investigated using an immunohistochemical method for the labeling of multiple antigens. About 50% of the cortical Lewy bodies in the cases with DLB were immunoreactive for clusterin, whereas brain-stem Lewy bodies in PD and DLB were rarely associated with clusterin. Clusterin was also immunopositive in around 10% of the glial cytoplasmic inclusions (GCIs) in the cases with MSA. Colocalization of clusterin with α-synuclein in such bodies or inclusions was clearly correlated with the immunostaining pattern of α-synuclein. Subcellular localization of clusterin was almost completely overlapped with the homogeneous immunoreaction of α-synuclein in the cortical Lewy bodies; however, clusterin immunoreactivity was not detected in the halo or ring-like structures of the brain-stem Lewy bodies. Furthermore, some Lewy bodies with intense immunoreactivity for clusterin showed only a weak signal for α-synuclein. These results suggest that clusterin may modify the formation of α-synuclein-positive inclusion bodies such as Lewy bodies and GCIs, through a previously proposed chaperone property of clusterin.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalActa neuropathologica
Volume104
Issue number3
Publication statusPublished - Dec 1 2002

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Clusterin
Lewy Bodies
Synucleins
Inclusion Bodies
Lewy Body Disease
Multiple System Atrophy
Neuroglia
Brain Stem
Parkinson Disease

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Clusterin/apolipoprotein J is associated with cortical Lewy bodies : Immunohistochemical study in cases with α-synucleinopathies. / Sasaki, Kensuke; Doh-ura, Katsumi; Wakisaka, Yoshinobu; Iwaki, Toru.

In: Acta neuropathologica, Vol. 104, No. 3, 01.12.2002, p. 225-230.

Research output: Contribution to journalArticle

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