Co-Delivery of Cell-Wall-Forming enzymes in the same vesicle for coordinated fungal cell wall formation

Martin Schuster, Magdalena Martin-Urdiroz, Yujiro Higuchi, Christian Hacker, Sreedhar Kilaru, Sarah J. Gurr, Gero Steinberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fungal cells are surrounded by an extracellular cell wall. This complex matrix of proteins and polysaccharides protects against adverse stresses and determines the shape of fungal cells. The polysaccharides of the fungal wall include 1,3-β-glucan and chitin, which are synthesized by membrane-bound synthases at the growing cell tip. A hallmark of filamentous fungi is the class V chitin synthase, which carries a myosin-motor domain. In the corn smut fungus Ustilago maydis, the myosin-chitin synthase Mcs1 moves to the plasma membrane in secretory vesicles, being delivered by kinesin-1 and myosin-5. The myosin domain of Mcs1 enhances polar secretion by tethering vesicles at the site of exocytosis. It remains elusive, however, how other cell-wall-forming enzymes are delivered and how their activity is coordinated post secretion. Here, we show that the U. maydis class VII chitin synthase and 1,3-β-glucan synthase travel in Mcs1-containing vesicles, and that their apical secretion depends on Mcs1. Once in the plasma membrane, anchorage requires enzyme activity, which suggests co-synthesis of chitin and 1,3-β-glucan polysaccharides at sites of exocytosis. Thus, delivery of cell-wall-forming enzymes in Mcs1 vesicles ensures local foci of fungal cell wall formation.

Original languageEnglish
Article number16149
JournalNature Microbiology
Volume1
DOIs
Publication statusPublished - Aug 26 2016

Fingerprint

Chitin Synthase
Myosins
Cell Wall
Chitin
Glucans
Exocytosis
Enzymes
Polysaccharides
Fungal Polysaccharides
Fungi
Cell Membrane
Ustilago
Kinesin
Cell Shape
Secretory Vesicles
Zea mays
Membranes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

Cite this

Co-Delivery of Cell-Wall-Forming enzymes in the same vesicle for coordinated fungal cell wall formation. / Schuster, Martin; Martin-Urdiroz, Magdalena; Higuchi, Yujiro; Hacker, Christian; Kilaru, Sreedhar; Gurr, Sarah J.; Steinberg, Gero.

In: Nature Microbiology, Vol. 1, 16149, 26.08.2016.

Research output: Contribution to journalArticle

Schuster, Martin ; Martin-Urdiroz, Magdalena ; Higuchi, Yujiro ; Hacker, Christian ; Kilaru, Sreedhar ; Gurr, Sarah J. ; Steinberg, Gero. / Co-Delivery of Cell-Wall-Forming enzymes in the same vesicle for coordinated fungal cell wall formation. In: Nature Microbiology. 2016 ; Vol. 1.
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