Co-occurrence and high-performance liquid chromatographic separation of the glycosides of rhodeasapogenin and its analogs which differ in the f-ring structure

Kazumoto Miyahara, Kudo Keiko, Toshio Kawasaki

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16 Citations (Scopus)

Abstract

Two spirostanol glycosides (steroid saponins) R-3 (I) and R-8 (II), which were regarded, respectively, as pure 1-0-α-L-rhamnopyranosyl-(1-2)-β-D-xylopyranoside and 3-0-β-D-glucopyranoside of rhodeasapogenin(25S) (III), were shown by 13C-NMR to be accompanied by the corresponding glycosides of isorhodeasapogenin(25R) (IV) and conva-llamarogenin(Δ25(27)) (V). Qualitative and preparative separation by HPLC of the components of I and II were studied and successfully achieved by a combination of two methods. They consisted equally of not three but four compounds, which were isolated in the pure state. Three of them were identified as the glycosides of III (major), IV and V, while the fourth was considered to have the same sugar moiety as I or II combined with a new aglycone different from III - V in the F-ring structure. The methods were applied also to some commonly known sterod saponins.

Original languageEnglish
Pages (from-to)348-351
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume31
Issue number1
DOIs
Publication statusPublished - 1983

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Drug Discovery

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