Coagulation factor XII (Hageman factor) Washington D.C. Inactive factor XIIa results from Cys-571 → Ser substitution

T. Miyata, S. I. Kawabata, S. Iwanaga, I. Takahashi, B. Alving, H. Saito

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36 Citations (Scopus)

Abstract

Structural studies on a congenital abnormal coagulation factor XII (Hageman factor), factor XII Washington D.C., have been performed to identify the defect responsible for its lack of procoagulant activity. Amino acid sequence analysis of a tryptic peptide isolated from the abnormal factor XII indicated that Cys-571 (equivalent to Cys-220 in the chymotrypsin numbering system) had been replaced by serine. No other substitutions in the active-site triad - namely, His-393, Asp-442, and Ser-544 - were found. We propose that the Cys-571 → Ser replacement found in this factor XII variant destroys the formation of the disulfide linkage between Cys-540 and Cys-571, giving rise to an altered conformation of the active-site serine residue or the secondary substrate-binding site and, thus, leads to the loss of enzyme activity.

Original languageEnglish
Pages (from-to)8319-8322
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number21
DOIs
Publication statusPublished - 1989

All Science Journal Classification (ASJC) codes

  • General

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