Coat assembly directs v-SNARE concentration into synthetic COPII vesicles

Ken Matsuoka, Yasujiro Morimitsu, Koji Uchida, Randy Schekman

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

COPII proteins are required to create transport vesicles and to select cargo molecules for transit from the ER. A reconstituted liposome budding reaction was used to detect the capture and concentration of membrane-associated v-SNARE molecules into synthetic COPII vesicles. A novel glutathione-phosphatidyl-ethanolamine conjugate (Glut-PE) was synthesized and incorporated into chemically defined liposomes to provide binding sites for GST hybrid proteins. Large liposomes containing bound cytoplasmic domains of the v-SNAREs, Sec22p or Bos1p, or of the ER resident proteins, Sec12p and Ufe1p, were exposed to COPII proteins and GMP-PNP. v-SNAREs but not resident proteins were concentrated in synthetic COPII vesicles generated from donor liposomes. We conclude that COPII proteins are necessary and sufficient for cargo selection and vesicle morphogenesis.

Original languageEnglish
Pages (from-to)703-708
Number of pages6
JournalMolecular Cell
Volume2
Issue number5
DOIs
Publication statusPublished - Nov 1998
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this