Colchicine- and Oryzalin-induced Tetraploids in Ornamental Alocasia X amazonica hort.

Nguyen Thi Phuong Thao, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


A procedure for chromosome duplication of Alocasia Xamazonica (2n=28) was established by using colchicine and oryzalin treatments to shoot tips of in vitro grown plants. Regenerated plants were screened by flow cytometry and their chromosome number was verified by cytological analysis of root tips. In total, 53 of 654 plants regenerated after the treatments appeared to be tetraploids and 144 plants were mixoploids. The treatments with colchicine and oryzalin produced 9.0 and 7.3 % and tetraploids, respectively. A maximum of 20 % tetraploid was obtained by the treatment with 0.05 % colchicine for 72 hr. Leaves of tetraploids have the tendency to be rounder, broader and thicker.

Original languageEnglish
Pages (from-to)63-65
Number of pages3
JournalJournal of the Japanese Society for Horticultural Science
Issue number1
Publication statusPublished - Jan 2004

All Science Journal Classification (ASJC) codes

  • Horticulture
  • Plant Science


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