Cold exposure down-regulates zebrafish hematopoiesis

Kasem Kulkeaw, Tohru Ishitani, Takaaki Kanemaru, Suthat Fucharoen, Daisuke Sugiyama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Erythropoiesis is regulated such that a sufficient number of mature erythrocytes is produced. Down-regulation of erythropoiesis causes various types of anemia. Although some anemia-related genes have been identified, there are several types of anemic disease for which the molecular mechanisms are yet unclear, suggesting that unidentified genes in addition to the classical cytokine pathways play important roles in anemia. To address this issue, a new animal model for anemia is required. We established a reversible anemic model in zebrafish by keeping fish at 17 °C, a low water temperature. In zebrafish kidney marrow, expression of several genes encoding hematopoietic transcription factors (Runx1, scl, c-myb and GATA-2) and particularly erythropoiesis-related factors (klfd, hbaa1, ba1, GATA-1, EPO, and EPOr) was down-regulated, whereas myelopoiesis-related factors (csf1a and csf3) was up-regulated in low temperature conditions. We propose that this zebrafish model is useful to identify novel genes for hematopoiesis, particularly erythropoiesis.

Original languageEnglish
Pages (from-to)859-864
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume394
Issue number4
DOIs
Publication statusPublished - Apr 16 2010

Fingerprint

Erythropoiesis
Hematopoiesis
Zebrafish
Anemia
Down-Regulation
Genes
Gene encoding
Myelopoiesis
Fish
Erythrocyte Count
Temperature
Animals
Transcription Factors
Cytokines
Fishes
Animal Models
Water
Bone Marrow
Kidney
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cold exposure down-regulates zebrafish hematopoiesis. / Kulkeaw, Kasem; Ishitani, Tohru; Kanemaru, Takaaki; Fucharoen, Suthat; Sugiyama, Daisuke.

In: Biochemical and Biophysical Research Communications, Vol. 394, No. 4, 16.04.2010, p. 859-864.

Research output: Contribution to journalArticle

Kulkeaw, Kasem ; Ishitani, Tohru ; Kanemaru, Takaaki ; Fucharoen, Suthat ; Sugiyama, Daisuke. / Cold exposure down-regulates zebrafish hematopoiesis. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 394, No. 4. pp. 859-864.
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