Cold exposure increases slow-type myosin heavy chain 1 (MyHC1) composition of soleus muscle in rats

Wataru Mizunoya, Yohei Iwamoto, Yusuke Sato, Ryuichi Tatsumi, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to examine the effects of cold exposure on rat skeletal muscle fiber type, according to myosin heavy chain (MyHC) isoform and metabolism-related factors. Male Wistar rats (7 weeks old) were housed individually at 4±2°C as a cold-exposed group or at room temperature (22±2°C) as a control group for 4 weeks. We found that cold exposure significantly increased the slow-type MyHC1 content in the soleus muscle (a typical slow-type fiber), while the intermediate-type MyHC2A content was significantly decreased. In contrast to soleus, MyHC composition of extensor digitorum longus (EDL, a typical fast-type fiber) and gastrocnemius (a mix of slow-type and fast-type fibers) muscle did not change from cold exposure. Cold exposure increased mRNA expression of mitochondrial uncoupling protein 3 (UCP3) in both the soleus and EDL. Cold exposure also increased mRNA expression of myoglobin, peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) and forkhead box O1 (FOXO1) in the soleus. Upregulation of UCP3 and PGC1α proteins were observed with Western blotting in the gastrocnemius. Thus, cold exposure increased metabolism-related factors in all muscle types that were tested, but MyHC isoforms changed only in the soleus.

Original languageEnglish
Pages (from-to)293-304
Number of pages12
JournalAnimal Science Journal
Volume85
Issue number3
DOIs
Publication statusPublished - Mar 1 2014

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Myosin Heavy Chains
myosin heavy chains
Skeletal Muscle
muscles
rats
Protein Isoforms
Muscles
Messenger RNA
metabolism
proteins
myoglobin
Myoglobin
Skeletal Muscle Fibers
muscle fibers
Wistar Rats
ambient temperature
Western blotting
Up-Regulation
Western Blotting
Control Groups

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Cold exposure increases slow-type myosin heavy chain 1 (MyHC1) composition of soleus muscle in rats. / Mizunoya, Wataru; Iwamoto, Yohei; Sato, Yusuke; Tatsumi, Ryuichi; Ikeuchi, Yoshihide.

In: Animal Science Journal, Vol. 85, No. 3, 01.03.2014, p. 293-304.

Research output: Contribution to journalArticle

Mizunoya, Wataru ; Iwamoto, Yohei ; Sato, Yusuke ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide. / Cold exposure increases slow-type myosin heavy chain 1 (MyHC1) composition of soleus muscle in rats. In: Animal Science Journal. 2014 ; Vol. 85, No. 3. pp. 293-304.
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