Collagen mimetic peptide engineered M13 bacteriophage for collagen targeting and imaging in cancer

Hyo Eon Jin, Rebecca Farr, Seung Wuk Lee

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Collagens are over-expressed in various human cancers and subsequently degraded and denatured by proteolytic enzymes, thus making them a target for diagnostics and therapeutics. Genetically engineered bacteriophage (phage) is a promising candidate for the development of imaging or therapeutic materials for cancer collagen targeting due to its promising structural features. We genetically engineered M13 phages with two functional peptides, collagen mimetic peptide and streptavidin binding peptide, on their minor and major coat proteins, respectively. The resulting engineered phage functions as a therapeutic or imaging material to target degraded and denatured collagens in cancerous tissues. We demonstrated that the engineered phages are able to target and label abnormal collagens expressed on A549 human lung adenocarcinoma cells after the conjugation with streptavidin-linked fluorescent agents. Our engineered collagen binding phage could be a useful platform for abnormal collagen imaging and drug delivery in various collagen-related diseases.

Original languageEnglish
Pages (from-to)9236-9245
Number of pages10
JournalBiomaterials
Volume35
Issue number33
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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