Abstract
Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.
| Original language | English |
|---|---|
| Pages (from-to) | 285-291 |
| Number of pages | 7 |
| Journal | Phytopathology |
| Volume | 90 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science
Cite this
Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves. / Yoshida, S.; Hiradate, Syuntaro; Fujii, Y.; Shirata, A.
In: Phytopathology, Vol. 90, No. 3, 01.01.2000, p. 285-291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves
AU - Yoshida, S.
AU - Hiradate, Syuntaro
AU - Fujii, Y.
AU - Shirata, A.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.
AB - Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.
UR - http://www.scopus.com/inward/record.url?scp=0033997018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033997018&partnerID=8YFLogxK
U2 - 10.1094/PHYTO.2000.90.3.285
DO - 10.1094/PHYTO.2000.90.3.285
M3 - Article
VL - 90
SP - 285
EP - 291
JO - Phytopathology
JF - Phytopathology
SN - 0031-949X
IS - 3
ER -