Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves

S. Yoshida, Syuntaro Hiradate, Y. Fujii, A. Shirata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalPhytopathology
Volume90
Issue number3
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Fingerprint

Colletotrichum dematium
lesions (plant)
phytotoxins
anthracnose
mulberries
leaves
fungi
toxins
Plantae
necrosis
extracts
toxic substances
chemical structure
acetone
pathogenesis

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves. / Yoshida, S.; Hiradate, Syuntaro; Fujii, Y.; Shirata, A.

In: Phytopathology, Vol. 90, No. 3, 01.01.2000, p. 285-291.

Research output: Contribution to journalArticle

Yoshida, S. ; Hiradate, Syuntaro ; Fujii, Y. ; Shirata, A. / Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves. In: Phytopathology. 2000 ; Vol. 90, No. 3. pp. 285-291.
@article{71f928cb443a43a8b68c31321daf2084,
title = "Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves",
abstract = "Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.",
author = "S. Yoshida and Syuntaro Hiradate and Y. Fujii and A. Shirata",
year = "2000",
month = "1",
day = "1",
doi = "10.1094/PHYTO.2000.90.3.285",
language = "English",
volume = "90",
pages = "285--291",
journal = "Phytopathology",
issn = "0031-949X",
publisher = "American Phytopathological Society",
number = "3",

}

TY - JOUR

T1 - Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves

AU - Yoshida, S.

AU - Hiradate, Syuntaro

AU - Fujii, Y.

AU - Shirata, A.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.

AB - Colletotrichum dematium, the causal agent of mulberry anthracnose, was examined to produce phytotoxins in vitro and in planta. Raw and autoclaved mulberry leaves infected with the fungus, as well as the fungus incubated with several solid or liquid media, were extracted with acetone. Extracts obtained from the fungus grown on raw and autoclaved mulberry leaves caused brown necrotic lesions on susceptible mulberry leaves when they were placed (10 μl) on the wounded adaxial surface. Whereas, no extracts obtained from media, except inoculated medium containing homogenized mulberry leaves, induced the necrosis, suggesting that the fungus produced phytotoxins in planta and that some components in mulberry leaves may be indispensable substrates for producing the toxins. The phytotoxins obtained from the diseased leaves induced necrosis on nonhost plants leaves as well as on mulberry leaves. The toxins were present in the border of anthracnose lesions on the leaves, and the sensitivity to the toxin correlated with that to the fungus infection in each susceptible or resistant mulberry cultivar. These results suggest that the phytotoxins are host nonspecific and play a role in fungal pathogenesis in the development of the lesions. Four toxic compounds were isolated and purified from anthracnose lesions. However, due to the low yield, the chemical structure of the compounds could not be identified.

UR - http://www.scopus.com/inward/record.url?scp=0033997018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033997018&partnerID=8YFLogxK

U2 - 10.1094/PHYTO.2000.90.3.285

DO - 10.1094/PHYTO.2000.90.3.285

M3 - Article

VL - 90

SP - 285

EP - 291

JO - Phytopathology

JF - Phytopathology

SN - 0031-949X

IS - 3

ER -