Colloidal nanoparticles from Poly(N-isopropylacrylamide)-graft-DNA for single nucleotide discrimination based on salt-induced aggregation

Extension to long target DNA

Zhonglan Tang, Tohru Takarada, Yoshikuni Sato, Mizuo Maeda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The DNA-linked colloidal nanoparticle was prepared through the phase transition of the amphiphilic copolymer consisting of DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacrylamide) as the hydrophobic part. We have succeeded in detecting the single nucleotide difference of 39-mer target DNA by using a salt-induced aggregation of the particles.

Original languageEnglish
Pages (from-to)1602-1603
Number of pages2
JournalChemistry Letters
Volume33
Issue number12
DOIs
Publication statusPublished - Dec 5 2004
Externally publishedYes

Fingerprint

Grafts
Agglomeration
Nucleotides
Salts
Nanoparticles
DNA
Copolymers
Phase transitions
poly-N-isopropylacrylamide

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Colloidal nanoparticles from Poly(N-isopropylacrylamide)-graft-DNA for single nucleotide discrimination based on salt-induced aggregation : Extension to long target DNA. / Tang, Zhonglan; Takarada, Tohru; Sato, Yoshikuni; Maeda, Mizuo.

In: Chemistry Letters, Vol. 33, No. 12, 05.12.2004, p. 1602-1603.

Research output: Contribution to journalArticle

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