Colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants

Martin R. Schroeder, Olga N. Borkhsenious, Ken Matsuoka, Kenzo Nakamura, Natasha V. Raikhel

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Various targeting motifs have been identified for plant proteins delivered to the vacuole. For barley (Hordeum vulgare) lectin, a typical Gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal propeptide. In contrast, the vacuolar targeting information of sporamin, a storage protein from the tuberous roots of the sweet potato (Ipomoea batatas), is encoded in an amino-terminal propeptide. Both proteins were expressed simultaneously in transgenic tobacco plants to enable analysis of their posttranslational processing and subcellular localization by pulse-chase labeling and electron-microscopic immunocytochemical methods. The pulse-chase experiments demonstrated that processing and delivery to the vacuole are not impaired by the simultaneous expression of barley lectin and sporamin. Both proteins were targeted quantitatively to the vacuole, indicating that the carboxyl-terminal and amino-terminal propeptides are equally recognized by the vacuolar protein-sorting machinery. Double-labeling experiments showed that barley lectin and sporamin accumulate in the same vacuole of transgenic tobacco (Nicotiana tabacum) leaf and root cells.

Original languageEnglish
Pages (from-to)451-458
Number of pages8
JournalPlant physiology
Volume101
Issue number2
DOIs
Publication statusPublished - Feb 1993

Fingerprint

sporamin
Genetically Modified Plants
Vacuoles
lectins
Tobacco
vacuoles
tobacco
barley
genetically modified organisms
Ipomoea batatas
Hordeum
Lectins
Proteins
Plant Proteins
protein transport
proteins
Protein Transport
storage proteins
sweet potatoes
plant proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants. / Schroeder, Martin R.; Borkhsenious, Olga N.; Matsuoka, Ken; Nakamura, Kenzo; Raikhel, Natasha V.

In: Plant physiology, Vol. 101, No. 2, 02.1993, p. 451-458.

Research output: Contribution to journalArticle

Schroeder, MR, Borkhsenious, ON, Matsuoka, K, Nakamura, K & Raikhel, NV 1993, 'Colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants', Plant physiology, vol. 101, no. 2, pp. 451-458. https://doi.org/10.1104/pp.101.2.451
Schroeder, Martin R. ; Borkhsenious, Olga N. ; Matsuoka, Ken ; Nakamura, Kenzo ; Raikhel, Natasha V. / Colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants. In: Plant physiology. 1993 ; Vol. 101, No. 2. pp. 451-458.
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