Colocalization of nitric oxide synthase, vasoactive intestinal polypeptide and tyrosine hydroxylase immunoreactivities in postganglionic neurons of the quail superior cervical ganglion

Katsuhito Itoh, Yasuhito Takaki, Koichi Ando, Tomoki Soh, Yasutoshi Ichinomiya, Haruo Kusaba

Research output: Contribution to journalArticle

Abstract

The colocalization of immunoreactivity to nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH) in the superior cervical ganglion (SCG) was investigated in the quail. In this bird, a substantial amount of NOS-immunoreactive (IR) cells were consistently found in the SCG without colchicine treatment or nerve ligation. The finding worthy of pointing out was that three-fourths of these NOS-IR cells were positive for TH. VIP-IR cells appeared with markedly low frequency than NOS-IR cells. They were generally small in size and often located in the ganglion peripheral. There were no VIP-IR cells positive for TH or negative for NOS: VIP immunoreactivity always appears in NOS-IR cells negative for TH. Thus, the results of the present study clearly showed the existence of two distinct subpopulations of postganglionic NOS-IR neurons (one is catecholaminergic and negative for VIP, and the other is non-catecholaminergic and positive for VIP). This suggests that nitric oxide (NO) and possibly VIP act as postganglionic neurotransmitters or neuromodulators in the quail SCG. The predominant appearance of the former category of NOS-IR cells must be considered in relation to some specific NO-induced controlling mechanisms of SCG neurons.

Original languageEnglish
Pages (from-to)439-443
Number of pages5
JournalJournal of Veterinary Medical Science
Volume75
Issue number4
DOIs
Publication statusPublished - May 10 2013

Fingerprint

tyrosine 3-monooxygenase
vasoactive intestinal peptide
Superior Cervical Ganglion
Quail
Vasoactive Intestinal Peptide
Tyrosine 3-Monooxygenase
quails
nitric oxide synthase
Nitric Oxide Synthase
neurons
Neurons
cells
neurotransmitters
nitric oxide
Neurotransmitter Agents
Nitric Oxide
colchicine
Colchicine
Ganglia
Birds

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Colocalization of nitric oxide synthase, vasoactive intestinal polypeptide and tyrosine hydroxylase immunoreactivities in postganglionic neurons of the quail superior cervical ganglion. / Itoh, Katsuhito; Takaki, Yasuhito; Ando, Koichi; Soh, Tomoki; Ichinomiya, Yasutoshi; Kusaba, Haruo.

In: Journal of Veterinary Medical Science, Vol. 75, No. 4, 10.05.2013, p. 439-443.

Research output: Contribution to journalArticle

@article{51498e55a5ad48fdb22772ec3f8ed5c3,
title = "Colocalization of nitric oxide synthase, vasoactive intestinal polypeptide and tyrosine hydroxylase immunoreactivities in postganglionic neurons of the quail superior cervical ganglion",
abstract = "The colocalization of immunoreactivity to nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH) in the superior cervical ganglion (SCG) was investigated in the quail. In this bird, a substantial amount of NOS-immunoreactive (IR) cells were consistently found in the SCG without colchicine treatment or nerve ligation. The finding worthy of pointing out was that three-fourths of these NOS-IR cells were positive for TH. VIP-IR cells appeared with markedly low frequency than NOS-IR cells. They were generally small in size and often located in the ganglion peripheral. There were no VIP-IR cells positive for TH or negative for NOS: VIP immunoreactivity always appears in NOS-IR cells negative for TH. Thus, the results of the present study clearly showed the existence of two distinct subpopulations of postganglionic NOS-IR neurons (one is catecholaminergic and negative for VIP, and the other is non-catecholaminergic and positive for VIP). This suggests that nitric oxide (NO) and possibly VIP act as postganglionic neurotransmitters or neuromodulators in the quail SCG. The predominant appearance of the former category of NOS-IR cells must be considered in relation to some specific NO-induced controlling mechanisms of SCG neurons.",
author = "Katsuhito Itoh and Yasuhito Takaki and Koichi Ando and Tomoki Soh and Yasutoshi Ichinomiya and Haruo Kusaba",
year = "2013",
month = "5",
day = "10",
doi = "10.1292/jvms.12-0400",
language = "English",
volume = "75",
pages = "439--443",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "4",

}

TY - JOUR

T1 - Colocalization of nitric oxide synthase, vasoactive intestinal polypeptide and tyrosine hydroxylase immunoreactivities in postganglionic neurons of the quail superior cervical ganglion

AU - Itoh, Katsuhito

AU - Takaki, Yasuhito

AU - Ando, Koichi

AU - Soh, Tomoki

AU - Ichinomiya, Yasutoshi

AU - Kusaba, Haruo

PY - 2013/5/10

Y1 - 2013/5/10

N2 - The colocalization of immunoreactivity to nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH) in the superior cervical ganglion (SCG) was investigated in the quail. In this bird, a substantial amount of NOS-immunoreactive (IR) cells were consistently found in the SCG without colchicine treatment or nerve ligation. The finding worthy of pointing out was that three-fourths of these NOS-IR cells were positive for TH. VIP-IR cells appeared with markedly low frequency than NOS-IR cells. They were generally small in size and often located in the ganglion peripheral. There were no VIP-IR cells positive for TH or negative for NOS: VIP immunoreactivity always appears in NOS-IR cells negative for TH. Thus, the results of the present study clearly showed the existence of two distinct subpopulations of postganglionic NOS-IR neurons (one is catecholaminergic and negative for VIP, and the other is non-catecholaminergic and positive for VIP). This suggests that nitric oxide (NO) and possibly VIP act as postganglionic neurotransmitters or neuromodulators in the quail SCG. The predominant appearance of the former category of NOS-IR cells must be considered in relation to some specific NO-induced controlling mechanisms of SCG neurons.

AB - The colocalization of immunoreactivity to nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH) in the superior cervical ganglion (SCG) was investigated in the quail. In this bird, a substantial amount of NOS-immunoreactive (IR) cells were consistently found in the SCG without colchicine treatment or nerve ligation. The finding worthy of pointing out was that three-fourths of these NOS-IR cells were positive for TH. VIP-IR cells appeared with markedly low frequency than NOS-IR cells. They were generally small in size and often located in the ganglion peripheral. There were no VIP-IR cells positive for TH or negative for NOS: VIP immunoreactivity always appears in NOS-IR cells negative for TH. Thus, the results of the present study clearly showed the existence of two distinct subpopulations of postganglionic NOS-IR neurons (one is catecholaminergic and negative for VIP, and the other is non-catecholaminergic and positive for VIP). This suggests that nitric oxide (NO) and possibly VIP act as postganglionic neurotransmitters or neuromodulators in the quail SCG. The predominant appearance of the former category of NOS-IR cells must be considered in relation to some specific NO-induced controlling mechanisms of SCG neurons.

UR - http://www.scopus.com/inward/record.url?scp=84877137959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877137959&partnerID=8YFLogxK

U2 - 10.1292/jvms.12-0400

DO - 10.1292/jvms.12-0400

M3 - Article

C2 - 23171690

AN - SCOPUS:84877137959

VL - 75

SP - 439

EP - 443

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 4

ER -