TY - JOUR
T1 - Combination use of anti-CD133 antibody and SSA lectin can effectively enrich cells with high tumorigenicity
AU - Moriwaki, Kenta
AU - Okudo, Kumiko
AU - Haraguchi, Naotsugu
AU - Takeishi, Shunsaku
AU - Sawaki, Hiromichi
AU - Narimatsu, Hisashi
AU - Tanemura, Masahiro
AU - Ishii, Hideshi
AU - Mori, Masaki
AU - Miyoshi, Eiji
PY - 2011/6
Y1 - 2011/6
N2 - Glycans exhibit characteristic changes in their structures during development and thus have been used as markers for stem/progenitor cells. However, the glycan structures unique to cancer stem cells (CSC) remain unknown. In the present study, we examined glycan structures in CD133+CD13+ CSC, which were recently found to have a high CSC ability, by means of a lectin microarray. Seven sialylated glycan-recognizing lectins, MAL-I, SNA, SSA, TJA-I, ACG, ABA and MAH, showed higher affinity to CD133+CD13+ CSC than CD133+ cells with a lower CSC ability. In addition, we demonstrated that CD133+SSA+ cells isolated from Huh7 cells had a significantly higher ability to form tumors in non-obese diabetic/severe combined immunodeficiency disease (NOD/SCID) mice and spheres under serum-free conditions than CD133+SSA- cells. These results suggest that hepatic CSC highly express sialylated glycans and that SSA lectin can be used as a tool for isolating CSC. This study is the first report to demonstrate the characteristic glycan structures in CSC and to indicate a new methodology involving lectins for isolating CSC.
AB - Glycans exhibit characteristic changes in their structures during development and thus have been used as markers for stem/progenitor cells. However, the glycan structures unique to cancer stem cells (CSC) remain unknown. In the present study, we examined glycan structures in CD133+CD13+ CSC, which were recently found to have a high CSC ability, by means of a lectin microarray. Seven sialylated glycan-recognizing lectins, MAL-I, SNA, SSA, TJA-I, ACG, ABA and MAH, showed higher affinity to CD133+CD13+ CSC than CD133+ cells with a lower CSC ability. In addition, we demonstrated that CD133+SSA+ cells isolated from Huh7 cells had a significantly higher ability to form tumors in non-obese diabetic/severe combined immunodeficiency disease (NOD/SCID) mice and spheres under serum-free conditions than CD133+SSA- cells. These results suggest that hepatic CSC highly express sialylated glycans and that SSA lectin can be used as a tool for isolating CSC. This study is the first report to demonstrate the characteristic glycan structures in CSC and to indicate a new methodology involving lectins for isolating CSC.
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U2 - 10.1111/j.1349-7006.2011.01923.x
DO - 10.1111/j.1349-7006.2011.01923.x
M3 - Article
C2 - 21392166
AN - SCOPUS:79956209666
VL - 102
SP - 1164
EP - 1170
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 6
ER -