Abstract
Tributyltin (TBT) and polychlorinated biphenyls (PCBs) are typical organic pollutants that co-exist in the aquatic environment. Despite increasing awareness of their combined impacts, knowledge about their potential interactive effects remains limited. In this study, we investigated the effects of TBT-PCBs mixtures on the survival, development, and hatching of embryos of Japanese medaka (Oryzias latipes). Our goal was to assess the interactive effects of TBT and PCBs on early-life-stage fish. We used in ovo nanoinjection to simulate the maternal transfer process and to control exposure doses at 0, 7.5, 15, 30 ng/g-egg (for both toxins). A factorial design (4 × 4) corresponding to the above doses was used to define the formulation of mixtures. Results showed that injection of mixtures of TBT and PCBs into fertilized eggs caused significantly elevated rates of mortality, yolk-sac shrinkage, swim-up failure, and deformities of medaka embryos/larvae. TBT and PCBs interacted synergistically to induce the above developmental abnormalities. In contrast, exposure to only TBT increased the hatching times of embryos, whereas exposure to only PCBs decreased the hatching times. The dose ratios, dose levels, and interactive effect of TBT and PCBs in a mixture played significant roles in determining the final impact on time-to-hatching. Our results suggested that in ovo exposure to a mixture of TBT and PCBs may interact synergistically to induce developmental abnormalities in fishes.
| Original language | English |
|---|---|
| Pages (from-to) | 927-934 |
| Number of pages | 8 |
| Journal | Chemosphere |
| Volume | 225 |
| DOIs | |
| Publication status | Published - Jun 1 2019 |
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All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Environmental Chemistry
- Chemistry(all)
- Pollution
- Health, Toxicology and Mutagenesis
Cite this
Combined toxicities of tributyltin and polychlorinated biphenyls on the development and hatching of Japanese medaka (Oryzias latipes) embryos via in ovo nanoinjection. / Qiu, Xuchun; Kim, Sang Gyoon; Kang, Ikjoon; Hano, Takeshi; Shimasaki, Yohei; Oshima, Yuji.
In: Chemosphere, Vol. 225, 01.06.2019, p. 927-934.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Combined toxicities of tributyltin and polychlorinated biphenyls on the development and hatching of Japanese medaka (Oryzias latipes) embryos via in ovo nanoinjection
AU - Qiu, Xuchun
AU - Kim, Sang Gyoon
AU - Kang, Ikjoon
AU - Hano, Takeshi
AU - Shimasaki, Yohei
AU - Oshima, Yuji
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Tributyltin (TBT) and polychlorinated biphenyls (PCBs) are typical organic pollutants that co-exist in the aquatic environment. Despite increasing awareness of their combined impacts, knowledge about their potential interactive effects remains limited. In this study, we investigated the effects of TBT-PCBs mixtures on the survival, development, and hatching of embryos of Japanese medaka (Oryzias latipes). Our goal was to assess the interactive effects of TBT and PCBs on early-life-stage fish. We used in ovo nanoinjection to simulate the maternal transfer process and to control exposure doses at 0, 7.5, 15, 30 ng/g-egg (for both toxins). A factorial design (4 × 4) corresponding to the above doses was used to define the formulation of mixtures. Results showed that injection of mixtures of TBT and PCBs into fertilized eggs caused significantly elevated rates of mortality, yolk-sac shrinkage, swim-up failure, and deformities of medaka embryos/larvae. TBT and PCBs interacted synergistically to induce the above developmental abnormalities. In contrast, exposure to only TBT increased the hatching times of embryos, whereas exposure to only PCBs decreased the hatching times. The dose ratios, dose levels, and interactive effect of TBT and PCBs in a mixture played significant roles in determining the final impact on time-to-hatching. Our results suggested that in ovo exposure to a mixture of TBT and PCBs may interact synergistically to induce developmental abnormalities in fishes.
AB - Tributyltin (TBT) and polychlorinated biphenyls (PCBs) are typical organic pollutants that co-exist in the aquatic environment. Despite increasing awareness of their combined impacts, knowledge about their potential interactive effects remains limited. In this study, we investigated the effects of TBT-PCBs mixtures on the survival, development, and hatching of embryos of Japanese medaka (Oryzias latipes). Our goal was to assess the interactive effects of TBT and PCBs on early-life-stage fish. We used in ovo nanoinjection to simulate the maternal transfer process and to control exposure doses at 0, 7.5, 15, 30 ng/g-egg (for both toxins). A factorial design (4 × 4) corresponding to the above doses was used to define the formulation of mixtures. Results showed that injection of mixtures of TBT and PCBs into fertilized eggs caused significantly elevated rates of mortality, yolk-sac shrinkage, swim-up failure, and deformities of medaka embryos/larvae. TBT and PCBs interacted synergistically to induce the above developmental abnormalities. In contrast, exposure to only TBT increased the hatching times of embryos, whereas exposure to only PCBs decreased the hatching times. The dose ratios, dose levels, and interactive effect of TBT and PCBs in a mixture played significant roles in determining the final impact on time-to-hatching. Our results suggested that in ovo exposure to a mixture of TBT and PCBs may interact synergistically to induce developmental abnormalities in fishes.
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U2 - 10.1016/j.chemosphere.2019.03.104
DO - 10.1016/j.chemosphere.2019.03.104
M3 - Article
VL - 225
SP - 927
EP - 934
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
ER -