Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and basic fibroblast growth factor releasing microspheres enhances recovery after cerebral ischemia in rats

Dai Matsuse, Masaaki Kitada, Fumitaka Ogura, Shohei Wakao, Misaki Kohama, Jun-Ichi Kira, Yasuhiko Tabata, Mari Dezawa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Bone marrow stromal cells (MSCs) are a useful source of cells because of their abundant supply and few associated ethical problems. We have previously reported that neural progenitor cells (NS-MSCs) can be effectively induced from MSCs and differentiate into neurons to contribute to functional recovery when transplanted into the rat stroke model. In this study, we attempted to enhance the therapeutic effects of NS-MSCs with a collagen sponge and basic fibroblast growth factor (bFGF) releasing microspheres. NS-MSCs were generated from MSCs by transfection of Notch-1 intracellular domain followed by culturing the cells in a free-floating culture system. The resulting NS-MSCs were transplanted into the rats with induced brain ischemia by using collagen sponges as scaffolds for transplanted cells, and with bFGF incorporated into gelatin microspheres to aid neovascularization around the transplanted region and proliferation of neural stem cells/neural progenitor cells. In culture, NS-MSCs successfully formed spheres containing cells highly expressing neural progenitor markers. Cell survival, neovascularization, and proliferation of host neural stem cells/neural progenitor cells were improved in animals that received NS-MSCs together with these biomaterials. Behavioral analysis also revealed significant functional recovery. These observations demonstrate that transplantation of NS-MSCs in combination with a collagen sponge and bFGF releasing microspheres significantly improves histological and functional recovery in the rat stroke model. When used with these biomaterials, NS-MSCs would be a promising cell source for treating stroke and neurodegenerative diseases.

Original languageEnglish
Pages (from-to)1993-2004
Number of pages12
JournalTissue Engineering - Part A
Volume17
Issue number15-16
DOIs
Publication statusPublished - Aug 1 2011

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Porifera
Fibroblast Growth Factor 2
Fibroblasts
Stromal Cells
Brain Ischemia
Microspheres
Mesenchymal Stromal Cells
Collagen
Rats
Bone
Stem Cells
Transplantation
Bone Marrow
Biocompatible Materials
Stem cells
Biomaterials
Recovery
Neurodegenerative diseases
Gelatin
Scaffolds (biology)

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and basic fibroblast growth factor releasing microspheres enhances recovery after cerebral ischemia in rats. / Matsuse, Dai; Kitada, Masaaki; Ogura, Fumitaka; Wakao, Shohei; Kohama, Misaki; Kira, Jun-Ichi; Tabata, Yasuhiko; Dezawa, Mari.

In: Tissue Engineering - Part A, Vol. 17, No. 15-16, 01.08.2011, p. 1993-2004.

Research output: Contribution to journalArticle

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