Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods

R. Nishino, K. Mikami, H. Takahashi, S. Tomonaga, Mitsuhiro Furuse, T. Hiramoto, Y. Aiba, Y. Koga, Nobuyuki Sudo

Research output: Contribution to journalArticle

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Abstract

Background: There is increasing evidence suggesting the existence of an interaction between commensal microbiota, the gut and the brain. The aim of this study was to examine the influence of commensal microbiota on the host behaviors in a contamination-free environment, which was verified by culture-based methods. Methods: Open-field and marble-burying tests were used to analyze anxiety-like behaviors and locomotor activity in gnotobiotic BALB/c mice with a common genetic background in a sterile isolator. The monoamine levels in several regions of the brain were measured in germfree (GF) mice and commensal fecal microbiota-associated mice (EX-GF). Key Results: A 24-h exposure to the environment outside the sterile isolators rendered GF mice less anxious than those not contaminated, while there was no change in the locomotion. EX-GF mice, the gnotobiotic mice with normal specific pathogen-free microbiota, were less anxious and active than GF mice using open-field and marble-burying tests. The norepinephrine, dopamine, and serotonin turnover rates were higher in the EX-GF mice than in the GF mice in most regions of the brain, suggesting that monoaminergic neurotransmission might increase in the EX-GF mice comparing the GF mice. Monoassociation with Brautia coccoides reduced the anxiety level, but it did not affect the locomotor activity. In contrast, colonization with Bifidobacterium infantis decreased the locomotor activity, while having little effect on the anxiety level. Conclusions & Inferences: These results strongly support the current view that gut microorganisms modulate brain development and behavior.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalNeurogastroenterology and Motility
Volume25
Issue number6
DOIs
Publication statusPublished - Jun 1 2013

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Microbiota
Locomotion
Germ-Free Life
Anxiety
Calcium Carbonate
Brain
Specific Pathogen-Free Organisms
Synaptic Transmission
Dopamine
Serotonin
Norepinephrine

All Science Journal Classification (ASJC) codes

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods. / Nishino, R.; Mikami, K.; Takahashi, H.; Tomonaga, S.; Furuse, Mitsuhiro; Hiramoto, T.; Aiba, Y.; Koga, Y.; Sudo, Nobuyuki.

In: Neurogastroenterology and Motility, Vol. 25, No. 6, 01.06.2013, p. 521-528.

Research output: Contribution to journalArticle

Nishino, R. ; Mikami, K. ; Takahashi, H. ; Tomonaga, S. ; Furuse, Mitsuhiro ; Hiramoto, T. ; Aiba, Y. ; Koga, Y. ; Sudo, Nobuyuki. / Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods. In: Neurogastroenterology and Motility. 2013 ; Vol. 25, No. 6. pp. 521-528.
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