Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells

Masakazu Namihira, Jun Kohyama, Katsunori Semi, Tsukasa Sanosaka, Benjamin Deneen, Tetsuya Taga, Kinichi Nakashima

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

During midgestation, mammalian neural precursor cells (NPCs) differentiate only into neurons. Generation of astrocytes is prevented at this stage, because astrocyte-specific gene promoters are methylated. How the subsequent switch from suppression to expression of astrocytic genes occurs is unknown. We show in this study that Notch ligands are expressed on committed neuronal precursors and young neurons in mid-gestational telencephalon, and that neighboring Notch-activated NPCs acquire the potential to become astrocytes. Activation of the Notch signaling pathway in midgestational NPCs induces expression of the transcription factor nuclear factor I, which binds to astrocytic gene promoters, resulting in demethylation of astrocyte-specific genes. These findings provide a mechanistic explanation for why neurons come first: committed neuronal precursors and young neurons potentiate remaining NPCs to differentiate into the next cell lineage, astrocytes.

Original languageEnglish
Pages (from-to)245-255
Number of pages11
JournalDevelopmental Cell
Volume16
Issue number2
DOIs
Publication statusPublished - Feb 17 2009
Externally publishedYes

Fingerprint

Astrocytes
Neurons
Genes
NFI Transcription Factors
Telencephalon
Cell Lineage
Transcription Factors
Chemical activation
Switches
Ligands
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells. / Namihira, Masakazu; Kohyama, Jun; Semi, Katsunori; Sanosaka, Tsukasa; Deneen, Benjamin; Taga, Tetsuya; Nakashima, Kinichi.

In: Developmental Cell, Vol. 16, No. 2, 17.02.2009, p. 245-255.

Research output: Contribution to journalArticle

Namihira, Masakazu ; Kohyama, Jun ; Semi, Katsunori ; Sanosaka, Tsukasa ; Deneen, Benjamin ; Taga, Tetsuya ; Nakashima, Kinichi. / Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells. In: Developmental Cell. 2009 ; Vol. 16, No. 2. pp. 245-255.
@article{eaf65c48d3b44399bf3fe807e18ba1a0,
title = "Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells",
abstract = "During midgestation, mammalian neural precursor cells (NPCs) differentiate only into neurons. Generation of astrocytes is prevented at this stage, because astrocyte-specific gene promoters are methylated. How the subsequent switch from suppression to expression of astrocytic genes occurs is unknown. We show in this study that Notch ligands are expressed on committed neuronal precursors and young neurons in mid-gestational telencephalon, and that neighboring Notch-activated NPCs acquire the potential to become astrocytes. Activation of the Notch signaling pathway in midgestational NPCs induces expression of the transcription factor nuclear factor I, which binds to astrocytic gene promoters, resulting in demethylation of astrocyte-specific genes. These findings provide a mechanistic explanation for why neurons come first: committed neuronal precursors and young neurons potentiate remaining NPCs to differentiate into the next cell lineage, astrocytes.",
author = "Masakazu Namihira and Jun Kohyama and Katsunori Semi and Tsukasa Sanosaka and Benjamin Deneen and Tetsuya Taga and Kinichi Nakashima",
year = "2009",
month = "2",
day = "17",
doi = "10.1016/j.devcel.2008.12.014",
language = "English",
volume = "16",
pages = "245--255",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells

AU - Namihira, Masakazu

AU - Kohyama, Jun

AU - Semi, Katsunori

AU - Sanosaka, Tsukasa

AU - Deneen, Benjamin

AU - Taga, Tetsuya

AU - Nakashima, Kinichi

PY - 2009/2/17

Y1 - 2009/2/17

N2 - During midgestation, mammalian neural precursor cells (NPCs) differentiate only into neurons. Generation of astrocytes is prevented at this stage, because astrocyte-specific gene promoters are methylated. How the subsequent switch from suppression to expression of astrocytic genes occurs is unknown. We show in this study that Notch ligands are expressed on committed neuronal precursors and young neurons in mid-gestational telencephalon, and that neighboring Notch-activated NPCs acquire the potential to become astrocytes. Activation of the Notch signaling pathway in midgestational NPCs induces expression of the transcription factor nuclear factor I, which binds to astrocytic gene promoters, resulting in demethylation of astrocyte-specific genes. These findings provide a mechanistic explanation for why neurons come first: committed neuronal precursors and young neurons potentiate remaining NPCs to differentiate into the next cell lineage, astrocytes.

AB - During midgestation, mammalian neural precursor cells (NPCs) differentiate only into neurons. Generation of astrocytes is prevented at this stage, because astrocyte-specific gene promoters are methylated. How the subsequent switch from suppression to expression of astrocytic genes occurs is unknown. We show in this study that Notch ligands are expressed on committed neuronal precursors and young neurons in mid-gestational telencephalon, and that neighboring Notch-activated NPCs acquire the potential to become astrocytes. Activation of the Notch signaling pathway in midgestational NPCs induces expression of the transcription factor nuclear factor I, which binds to astrocytic gene promoters, resulting in demethylation of astrocyte-specific genes. These findings provide a mechanistic explanation for why neurons come first: committed neuronal precursors and young neurons potentiate remaining NPCs to differentiate into the next cell lineage, astrocytes.

UR - http://www.scopus.com/inward/record.url?scp=59649112849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59649112849&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2008.12.014

DO - 10.1016/j.devcel.2008.12.014

M3 - Article

C2 - 19217426

AN - SCOPUS:59649112849

VL - 16

SP - 245

EP - 255

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 2

ER -