Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers.

C. Uematsu, J. Nishida, K. Okano, H. Kambara, F. Miura, T. Ito, Y. Sakaki

Research output: Contribution to journalArticle

Abstract

We have developed a method for comparative analysis of gene expression. It is based on competitive PCR amplification with module-shuffling primers, followed by gel electrophoresis in a fluorescent DNA sequencer. In this method, tagged-cDNA restriction fragments derived from different sources were amplified in one tube at the same amplification efficiency. The method can detect different amounts of each expressed gene, up to difference in amounts of 30%. The method was successfully used for comparative analysis of expressed genes in yeast.

Original languageEnglish
Pages (from-to)91-92
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Polymerase Chain Reaction
Genes
Electrophoresis
Complementary DNA
Yeasts
Gels
Gene Expression
DNA

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Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers. / Uematsu, C.; Nishida, J.; Okano, K.; Kambara, H.; Miura, F.; Ito, T.; Sakaki, Y.

In: Nucleic acids research. Supplement (2001), No. 1, 01.01.2001, p. 91-92.

Research output: Contribution to journalArticle

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