TY - JOUR
T1 - Comparative analysis of CreER transgenic mice for the study of brain macrophages
T2 - A case study
AU - Chappell-Maor, Louise
AU - Kolesnikov, Masha
AU - Kim, Jung Seok
AU - Shemer, Anat
AU - Haimon, Zhana
AU - Grozovski, Jonathan
AU - Boura-Halfon, Sigalit
AU - Masuda, Takahiro
AU - Prinz, Marco
AU - Jung, Steffen
N1 - Funding Information:
The Jung laboratory was supported by the Israel Science Foundation (887/11), the European Research Council (Adv ERC 340345), and a collaborative network grant of the International Progressive MS Alliance (PMSA). S.J. and M.P. were supported by the Deutsche Forschungsgemeinschaft (DFG) (CRC/TRR167 ‘NeuroMac’).
Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx3cr1CreER and Sall1CreER transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.
AB - Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx3cr1CreER and Sall1CreER transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.
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U2 - 10.1002/eji.201948342
DO - 10.1002/eji.201948342
M3 - Article
C2 - 31762013
AN - SCOPUS:85076825099
SN - 0014-2980
VL - 50
SP - 353
EP - 362
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 3
ER -