Comparative analysis of CreER transgenic mice for the study of brain macrophages: A case study

Louise Chappell-Maor, Masha Kolesnikov, Jung Seok Kim, Anat Shemer, Zhana Haimon, Jonathan Grozovski, Sigalit Boura-Halfon, Takahiro Masuda, Marco Prinz, Steffen Jung

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3 Citations (Scopus)

Abstract

Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx3cr1CreER and Sall1CreER transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.

Original languageEnglish
Pages (from-to)353-362
Number of pages10
JournalEuropean Journal of Immunology
Volume50
Issue number3
DOIs
Publication statusPublished - Mar 1 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Chappell-Maor, L., Kolesnikov, M., Kim, J. S., Shemer, A., Haimon, Z., Grozovski, J., Boura-Halfon, S., Masuda, T., Prinz, M., & Jung, S. (2020). Comparative analysis of CreER transgenic mice for the study of brain macrophages: A case study. European Journal of Immunology, 50(3), 353-362. https://doi.org/10.1002/eji.201948342