Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression

Takahiro Suzuki, Mai Khoi Q. Do, Yusuke Sato, Koichi Ojima, Minako Hara, Wataru Mizunoya, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, Judy E. Anderson, Ryuichi Tatsumi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalInternational Journal of Biochemistry and Cell Biology
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 1 2013

Fingerprint

Semaphorin-3A
Myogenin
Muscle Cells
Muscle
Satellites
Fibroblast Growth Factor 2
varespladib methyl
Neuropilin-2
Skeletal Muscle
Neuropilins
Skeletal Muscle Satellite Cells
Neuropilin-1
Muscles
Hepatocyte Growth Factor
Conditioned Culture Medium
Stem cells
Modulators
In Vitro Techniques
Rats
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology

Cite this

Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression. / Suzuki, Takahiro; Do, Mai Khoi Q.; Sato, Yusuke; Ojima, Koichi; Hara, Minako; Mizunoya, Wataru; Nakamura, Mako; Furuse, Mitsuhiro; Ikeuchi, Yoshihide; Anderson, Judy E.; Tatsumi, Ryuichi.

In: International Journal of Biochemistry and Cell Biology, Vol. 45, No. 2, 01.02.2013, p. 476-482.

Research output: Contribution to journalArticle

Suzuki, Takahiro ; Do, Mai Khoi Q. ; Sato, Yusuke ; Ojima, Koichi ; Hara, Minako ; Mizunoya, Wataru ; Nakamura, Mako ; Furuse, Mitsuhiro ; Ikeuchi, Yoshihide ; Anderson, Judy E. ; Tatsumi, Ryuichi. / Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression. In: International Journal of Biochemistry and Cell Biology. 2013 ; Vol. 45, No. 2. pp. 476-482.
@article{f82ceecbe622490b883af17d91d13bc7,
title = "Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression",
abstract = "Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.",
author = "Takahiro Suzuki and Do, {Mai Khoi Q.} and Yusuke Sato and Koichi Ojima and Minako Hara and Wataru Mizunoya and Mako Nakamura and Mitsuhiro Furuse and Yoshihide Ikeuchi and Anderson, {Judy E.} and Ryuichi Tatsumi",
year = "2013",
month = "2",
day = "1",
doi = "10.1016/j.biocel.2012.10.003",
language = "English",
volume = "45",
pages = "476--482",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression

AU - Suzuki, Takahiro

AU - Do, Mai Khoi Q.

AU - Sato, Yusuke

AU - Ojima, Koichi

AU - Hara, Minako

AU - Mizunoya, Wataru

AU - Nakamura, Mako

AU - Furuse, Mitsuhiro

AU - Ikeuchi, Yoshihide

AU - Anderson, Judy E.

AU - Tatsumi, Ryuichi

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.

AB - Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.

UR - http://www.scopus.com/inward/record.url?scp=84872311476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872311476&partnerID=8YFLogxK

U2 - 10.1016/j.biocel.2012.10.003

DO - 10.1016/j.biocel.2012.10.003

M3 - Article

C2 - 23085379

AN - SCOPUS:84872311476

VL - 45

SP - 476

EP - 482

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

SN - 1357-2725

IS - 2

ER -