TY - JOUR
T1 - Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression
AU - Suzuki, Takahiro
AU - Do, Mai Khoi Q.
AU - Sato, Yusuke
AU - Ojima, Koichi
AU - Hara, Minako
AU - Mizunoya, Wataru
AU - Nakamura, Mako
AU - Furuse, Mitsuhiro
AU - Ikeuchi, Yoshihide
AU - Anderson, Judy E.
AU - Tatsumi, Ryuichi
N1 - Funding Information:
This work was supported by Grants-in-Aid for Challenging Exploratory Research 20658067 , Scientific Research (B) 19380152 and 22380145 and Scientific Research (A) 24248045 , and by Invitation Fellowship Program for Research in Japan from the Japan Society for the Promotion of Science (JSPS) and by grant funds from Faculty of Agriculture, Kyushu University (all to R. Tatsumi). The research was also supported by funds from the Canadian Space Agency 9F007-52237-001-SR and the Natural Sciences and Engineering Research Council 171302 (to J.E. Anderson). T. Suzuki received a JSPS Pre-Doctoral Research fellowship for Young Scientists and Grant-in-Aid for JSPS Fellows. M.-K.Q. Do received a scholarship from the Ministry of Education, Culture, Sports, Science and Technology-Japan (MEXT) during the course of this research.
PY - 2013/2
Y1 - 2013/2
N2 - Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.
AB - Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.
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U2 - 10.1016/j.biocel.2012.10.003
DO - 10.1016/j.biocel.2012.10.003
M3 - Article
C2 - 23085379
AN - SCOPUS:84872311476
VL - 45
SP - 476
EP - 482
JO - International Journal of Biochemistry
JF - International Journal of Biochemistry
SN - 1357-2725
IS - 2
ER -