Comparative community gene expression analysis of Aquificales-dominated geothermal springs

Natsuko Hamamura, Jennifer Meneghin, Anna Louise Reysenbach

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Members of Sulfurihydrogenibium are often observed as visible filamentous biomass in circumneutral hot springs and play roles in sulfur-cycling, hydrogen oxidation and iron mineralization. To gain insight into the ecophysiology of Sulfurihydrogenibium populations, we conducted preliminary metatranscriptomic analysis of three distinct thermal springs; Calcite Springs (YNP-CS) and Mammoth Springs (YNP-MHS) in Yellowstone National Park, USA, and Furnas Springs (AZ) in Azores, Portugal. Genes to which transcripts were assigned revealed commonly expressed functions among the sites, while several differences were also observed. All three sites, Sulfurihydrogenibium spp. dominate and are obtaining energy via metabolism of sulfur compounds under microaerophilic conditions. Cell motility was one of the expressed functions in two sites (YNP-CS and AZ) with slower stream flow rates and thicker well-formed biofilms. The transcripts from YNP-CS and -MHS exhibited varying levels of sequence divergence from the reference genomes and corresponding metagenomes, suggesting the presence of microdiversity among Sulfurihydrogenibium populations in situ. Conversely, the majority of the AZ transcripts were identical to the S. azorense genome. Our initial results show that the metatranscriptomes in these similar Aquificales-dominated communities can reveal community-level gene function in geochemically distinct thermal environments.

Original languageEnglish
Pages (from-to)1226-1237
Number of pages12
JournalEnvironmental Microbiology
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 1 2013
Externally publishedYes

Fingerprint

Aquificales
Hot Springs
hot springs
thermal spring
gene expression
Azores
genome
Hot Temperature
Metagenome
Genome
Gene Expression
Sulfur Compounds
ecophysiology
Portugal
Calcium Carbonate
gene
sulfur compound
sulfur
motility
Biofilms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Comparative community gene expression analysis of Aquificales-dominated geothermal springs. / Hamamura, Natsuko; Meneghin, Jennifer; Reysenbach, Anna Louise.

In: Environmental Microbiology, Vol. 15, No. 4, 01.04.2013, p. 1226-1237.

Research output: Contribution to journalArticle

Hamamura, Natsuko ; Meneghin, Jennifer ; Reysenbach, Anna Louise. / Comparative community gene expression analysis of Aquificales-dominated geothermal springs. In: Environmental Microbiology. 2013 ; Vol. 15, No. 4. pp. 1226-1237.
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