Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection

Mostafa Abdelrahman, Naoyuki Suzumura, Mai Mitoma, Satoshi Matsuo, Takao Ikeuchi, Mitsutaka Mori, Kyoko Murakami, Yukio Ozaki, Masaru Matsumoto, Atsuko Uragami, Akira Kanno

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-Time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-Associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.

Original languageEnglish
Article number2608
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Diaporthe
Asparagus officinalis
transcriptome
asparagus spears
transcriptomics
infection
peroxidase
jasmonic acid
phospholipase D
stems
genes
pathogenesis-related proteins
wild relatives
methyl jasmonate
resistance mechanisms
genetic resources
blight
disease resistance
sequence analysis
reverse transcriptase polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • General

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Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection. / Abdelrahman, Mostafa; Suzumura, Naoyuki; Mitoma, Mai; Matsuo, Satoshi; Ikeuchi, Takao; Mori, Mitsutaka; Murakami, Kyoko; Ozaki, Yukio; Matsumoto, Masaru; Uragami, Atsuko; Kanno, Akira.

In: Scientific reports, Vol. 7, No. 1, 2608, 01.12.2017.

Research output: Contribution to journalArticle

Abdelrahman, Mostafa ; Suzumura, Naoyuki ; Mitoma, Mai ; Matsuo, Satoshi ; Ikeuchi, Takao ; Mori, Mitsutaka ; Murakami, Kyoko ; Ozaki, Yukio ; Matsumoto, Masaru ; Uragami, Atsuko ; Kanno, Akira. / Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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