Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

Junko Tomida; Ayako Oumi; Tatsuya Okamoto; Yuji Morita; Akihiko Okayama; Naoaki Misawa; Tetsuya Hayashi; Takaaki Akaike; Yoshiaki Kawamura

doi:10.1111/1348-0421.12044

Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

Junko Tomida, Ayako Oumi, Tatsuya Okamoto, Yuji Morita, Akihiko Okayama, Naoaki Misawa, Tetsuya Hayashi, Takaaki Akaike, Yoshiaki Kawamura

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

Original language	English
Pages (from-to)	353-358
Number of pages	6
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	57
Issue number	5
DOIs	https://doi.org/10.1111/1348-0421.12044
Publication status	Published - May 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Virology

Access to Document

10.1111/1348-0421.12044

Cite this

@article{2b74e60867f944c9b22c59e05fa9fe4f,

title = "Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi",

abstract = "The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.",

author = "Junko Tomida and Ayako Oumi and Tatsuya Okamoto and Yuji Morita and Akihiko Okayama and Naoaki Misawa and Tetsuya Hayashi and Takaaki Akaike and Yoshiaki Kawamura",

year = "2013",

month = may,

doi = "10.1111/1348-0421.12044",

language = "English",

volume = "57",

pages = "353--358",

journal = "Microbiology and Immunology",

issn = "0385-5600",

publisher = "Center for Academic Publications Japan",

number = "5",

}

TY - JOUR

T1 - Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

AU - Tomida, Junko

AU - Oumi, Ayako

AU - Okamoto, Tatsuya

AU - Morita, Yuji

AU - Okayama, Akihiko

AU - Misawa, Naoaki

AU - Hayashi, Tetsuya

AU - Akaike, Takaaki

AU - Kawamura, Yoshiaki

PY - 2013/5

Y1 - 2013/5

N2 - The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

AB - The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

UR - http://www.scopus.com/inward/record.url?scp=84877885457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877885457&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12044

DO - 10.1111/1348-0421.12044

M3 - Article

C2 - 23668607

AN - SCOPUS:84877885457

SN - 0385-5600

VL - 57

SP - 353

EP - 358

JO - Microbiology and Immunology

JF - Microbiology and Immunology

IS - 5

ER -

Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this