Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

Junko Tomida, Ayako Oumi, Tatsuya Okamoto, Yuji Morita, Akihiko Okayama, Naoaki Misawa, Tetsuya Hayashi, Takaaki Akaike, Yoshiaki Kawamura

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Abstract

The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

Original languageEnglish
Pages (from-to)353-358
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume57
Issue number5
DOIs
Publication statusPublished - May 1 2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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