Comparative proteomic analysis of two stress-management strategies in pancreatic cancer

Byron Baron, Tsuyoshi Fujioka, Takao Kitagawa, Shin Ichiro Maehara, Yoshihiko Maehara, Kazuyuki Nakamura, Yasuhiro Kuramitsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: It is known that cancers adopt different strategies to cope with stress and overcome adverse micro-environmental conditions. Such strategies are also applicable to chemo-therapeutic treatment, which could subsequently result in chemo-resistance. Materials and Methods: In order to investigate known stress-evasion strategies observed in pancreatic cancer, the stress-resistant KLM1-derived cell lines KLM1-R (Gemcitabine (GEM)-induced stress) and KLM1-S (growth factor restriction-induced stress) were employed. Comparative proteomics were employed between for the two cell lines that were also compared against the parent cell line KLM1. Results: Proteomic analysis revealed changes in the expression levels of 6 proteins, namely: transitional endoplasmic reticulum ATPase, lamin A/C, PDZ and LIM protein 1, calmodulin, heat shock protein 60 and alpha enolase. Resistance to GEM of KLM1-R and KLM1-S was found to be comparable, with KLM1-S cells exhibiting close to 1.5-fold higher half-maximal inhibitory concentration ( IC50) compared to KLM1-R cells. Conclusion: These results suggest that KLM1-R can be used as a model of directly-acquired chemoresistance (responding directly to evade GEM treatment), while KLM1-S is a good model of indirectly-acquired chemoresistance (formed in response to having to survive with less availability of growth factors), additionally gaining a selective advantage upon GEM treatment.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalCancer Genomics and Proteomics
Volume12
Issue number2
Publication statusPublished - Mar 1 2015

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gemcitabine
Pancreatic Neoplasms
Proteomics
Cell Line
Cells
Intercellular Signaling Peptides and Proteins
Lamin Type A
Chaperonin 60
Phosphopyruvate Hydratase
Calmodulin
Endoplasmic Reticulum
Inhibitory Concentration 50
Adenosine Triphosphatases
Proteins
Availability
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Baron, B., Fujioka, T., Kitagawa, T., Maehara, S. I., Maehara, Y., Nakamura, K., & Kuramitsu, Y. (2015). Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. Cancer Genomics and Proteomics, 12(2), 83-88.

Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. / Baron, Byron; Fujioka, Tsuyoshi; Kitagawa, Takao; Maehara, Shin Ichiro; Maehara, Yoshihiko; Nakamura, Kazuyuki; Kuramitsu, Yasuhiro.

In: Cancer Genomics and Proteomics, Vol. 12, No. 2, 01.03.2015, p. 83-88.

Research output: Contribution to journalArticle

Baron, B, Fujioka, T, Kitagawa, T, Maehara, SI, Maehara, Y, Nakamura, K & Kuramitsu, Y 2015, 'Comparative proteomic analysis of two stress-management strategies in pancreatic cancer', Cancer Genomics and Proteomics, vol. 12, no. 2, pp. 83-88.
Baron B, Fujioka T, Kitagawa T, Maehara SI, Maehara Y, Nakamura K et al. Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. Cancer Genomics and Proteomics. 2015 Mar 1;12(2):83-88.
Baron, Byron ; Fujioka, Tsuyoshi ; Kitagawa, Takao ; Maehara, Shin Ichiro ; Maehara, Yoshihiko ; Nakamura, Kazuyuki ; Kuramitsu, Yasuhiro. / Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. In: Cancer Genomics and Proteomics. 2015 ; Vol. 12, No. 2. pp. 83-88.
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