Comparative proteomic analysis of two stress-management strategies in pancreatic cancer

Byron Baron, Tsuyoshi Fujioka, Takao Kitagawa, Shin Ichiro Maehara, Yoshihiko Maehara, Kazuyuki Nakamura, Yasuhiro Kuramitsu

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4 Citations (Scopus)

Abstract

Background: It is known that cancers adopt different strategies to cope with stress and overcome adverse micro-environmental conditions. Such strategies are also applicable to chemo-therapeutic treatment, which could subsequently result in chemo-resistance. Materials and Methods: In order to investigate known stress-evasion strategies observed in pancreatic cancer, the stress-resistant KLM1-derived cell lines KLM1-R (Gemcitabine (GEM)-induced stress) and KLM1-S (growth factor restriction-induced stress) were employed. Comparative proteomics were employed between for the two cell lines that were also compared against the parent cell line KLM1. Results: Proteomic analysis revealed changes in the expression levels of 6 proteins, namely: transitional endoplasmic reticulum ATPase, lamin A/C, PDZ and LIM protein 1, calmodulin, heat shock protein 60 and alpha enolase. Resistance to GEM of KLM1-R and KLM1-S was found to be comparable, with KLM1-S cells exhibiting close to 1.5-fold higher half-maximal inhibitory concentration ( IC50) compared to KLM1-R cells. Conclusion: These results suggest that KLM1-R can be used as a model of directly-acquired chemoresistance (responding directly to evade GEM treatment), while KLM1-S is a good model of indirectly-acquired chemoresistance (formed in response to having to survive with less availability of growth factors), additionally gaining a selective advantage upon GEM treatment.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalCancer Genomics and Proteomics
Volume12
Issue number2
Publication statusPublished - Mar 1 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Baron, B., Fujioka, T., Kitagawa, T., Maehara, S. I., Maehara, Y., Nakamura, K., & Kuramitsu, Y. (2015). Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. Cancer Genomics and Proteomics, 12(2), 83-88.