Comparative proteomics uncovers the signature of natural selection acting on the ejaculate proteomes of two cricket species isolated by postmating, prezygotic phenotypes

Jeremy L. Marshall, Diana L. Huestis, Christopher Garcia, Yasuaki Hiromasa, Shanda Wheeler, Suegene Noh, John M. Tomich, Daniel J. Howard

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Two of the most well-supported patterns to have emerged over the past two decades of research in evolutionary biology are the occurrence of divergent natural selection acting on many male and female reproductive tract proteins and the importance of postmating, prezygotic phenotypes in reproductively isolating closely related species. Although these patterns appear to be common across a wide variety of taxa, the link between them remains poorly documented. Here, we utilize comparative proteomic techniques to determine whether or not there is evidence for natural selection acting on the ejaculate proteomes of two cricket species (Allonemobius fasciatus and A. socius) which are reproductively isolated primarily by postmating, prezygotic phenotypes. In addressing this question, we compare the degree of within-species polymorphism and between-species divergence between the ejaculate and thorax proteomes of these two species. We found that the ejaculate proteomes are both less polymorphic and more divergent than the thorax proteomes. Additionally, we assessed patterns of nucleotide variation for two species-specific ejaculate proteins and found evidence for both reduced levels of variation within species and positive selection driving divergence between species. In contrast, non-species-specific proteins exhibited higher levels of within-species nucleotide variation and no signatures of positive selection. Nucleotide and putative functional data for the two species-specific proteins, along with data for a third protein (ejaculate serine protease), suggest that all three of these genes are candidate speciation genes in need of further study. Overall, these patterns of proteome and nucleotide divergence provide support for the hypothesis that there is a causative link between selection-driven divergence of male ejaculate proteins and the evolution of postmating, prezygotic barriers to gene flow within Allonemobius.

Original languageEnglish
Pages (from-to)423-435
Number of pages13
JournalMolecular Biology and Evolution
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Fingerprint

Gryllidae
proteomics
cricket
Genetic Selection
Proteome
proteome
natural selection
Proteomics
phenotype
Phenotype
Nucleotides
nucleotides
Proteins
proteins
protein
thorax
divergence
Allonemobius
Allonemobius fasciatus
Allonemobius socius

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Comparative proteomics uncovers the signature of natural selection acting on the ejaculate proteomes of two cricket species isolated by postmating, prezygotic phenotypes. / Marshall, Jeremy L.; Huestis, Diana L.; Garcia, Christopher; Hiromasa, Yasuaki; Wheeler, Shanda; Noh, Suegene; Tomich, John M.; Howard, Daniel J.

In: Molecular Biology and Evolution, Vol. 28, No. 1, 01.01.2011, p. 423-435.

Research output: Contribution to journalArticle

Marshall, Jeremy L. ; Huestis, Diana L. ; Garcia, Christopher ; Hiromasa, Yasuaki ; Wheeler, Shanda ; Noh, Suegene ; Tomich, John M. ; Howard, Daniel J. / Comparative proteomics uncovers the signature of natural selection acting on the ejaculate proteomes of two cricket species isolated by postmating, prezygotic phenotypes. In: Molecular Biology and Evolution. 2011 ; Vol. 28, No. 1. pp. 423-435.
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