Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring

Anke Lange, Yoshinao Katsu, Shinichi Miyagawa, Yukiko Ogino, Hiroshi Urushitani, Tohru Kobayashi, Toshiaki Hirai, Janice A. Shears, Masaki Nagae, Jun Yamamoto, Yuta Ohnishi, Tomohiro Oka, Norihisa Tatarazako, Yasuhiko Ohta, Charles R. Tyler, Taisen Iguchi

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Exposure to estrogenic chemicals discharged into the aquatic environment has been shown to induce feminization in wild freshwater fish and although fish species have been reported to differ in their susceptibility for these effects, empirical studies that directly address this hypothesis are lacking. In this study, in vitro ERα activation assays were applied in a range of fish species used widely in chemical testing (including, zebrafish, fathead minnow, medaka) and/or as environmental monitoring species (including, roach, stickleback, carp) to assess their comparative responsiveness to natural (estrone, estradiol, estriol) and synthetic (17α-ethinylestradiol (EE2), diethylstilbestrol (DES)) estrogens. In vivo exposures to EE2 via the water (nominal 2 and 10. ng/L for 7 days) were also conducted for seven fish species to compare their responsiveness for hepatic vitellogenin (VTG) mRNA induction (an ER mediated response). Of the fish species tested, zebrafish ERα was found to be the most responsive and carp and stickleback ERα the least responsive to natural steroid estrogens. This was also the case for exposure to EE2 with an ERα-mediated response sensitivity order of zebrafish > medaka > roach > fathead minnow > carp > stickleback. For VTG mRNA induction in vivo, the order of species responsiveness was: rainbow trout (not tested in the ERα activation assays) > zebrafish > fathead minnow > medaka > roach > stickleback > carp. Overall, the responses to steroid estrogens in vitro via ERα compared well with those seen in vivo (VTG induction for exposure to EE2) showing in vitro screening of chemicals using fish ERα-mediated responses indicative of estrogenic responses (VTG induction) in vivo.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalAquatic Toxicology
Volume109
DOIs
Publication statusPublished - Mar 1 2012
Externally publishedYes

Fingerprint

synthetic estrogens
Estradiol Congeners
Smegmamorpha
Gasterosteidae
vitellogenin
Vitellogenins
Fishes
Danio rerio
Carps
carp
Pimephales promelas
Rutilus rutilus
Zebrafish
Oryzias
Cyprinidae
monitoring
estrogens
fish
Estrogens
steroids

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring. / Lange, Anke; Katsu, Yoshinao; Miyagawa, Shinichi; Ogino, Yukiko; Urushitani, Hiroshi; Kobayashi, Tohru; Hirai, Toshiaki; Shears, Janice A.; Nagae, Masaki; Yamamoto, Jun; Ohnishi, Yuta; Oka, Tomohiro; Tatarazako, Norihisa; Ohta, Yasuhiko; Tyler, Charles R.; Iguchi, Taisen.

In: Aquatic Toxicology, Vol. 109, 01.03.2012, p. 250-258.

Research output: Contribution to journalArticle

Lange, A, Katsu, Y, Miyagawa, S, Ogino, Y, Urushitani, H, Kobayashi, T, Hirai, T, Shears, JA, Nagae, M, Yamamoto, J, Ohnishi, Y, Oka, T, Tatarazako, N, Ohta, Y, Tyler, CR & Iguchi, T 2012, 'Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring', Aquatic Toxicology, vol. 109, pp. 250-258. https://doi.org/10.1016/j.aquatox.2011.09.004
Lange, Anke ; Katsu, Yoshinao ; Miyagawa, Shinichi ; Ogino, Yukiko ; Urushitani, Hiroshi ; Kobayashi, Tohru ; Hirai, Toshiaki ; Shears, Janice A. ; Nagae, Masaki ; Yamamoto, Jun ; Ohnishi, Yuta ; Oka, Tomohiro ; Tatarazako, Norihisa ; Ohta, Yasuhiko ; Tyler, Charles R. ; Iguchi, Taisen. / Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring. In: Aquatic Toxicology. 2012 ; Vol. 109. pp. 250-258.
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