Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases

Nobuo Maita, James Nyirenda, Mayumi Igura, Jun Kamishikiryo, Daisuke Kohda

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    Oligosaccharyltransferase (OST) catalyzes the transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. In the bacterium Campylobacter jejuni, a single-subunit membrane protein, PglB, catalyzes N-glycosylation. We report the 2.8 Å resolution crystal structure of the C-terminal globular domain of PglB and its comparison with the previously determined structure from the archaeon Pyrococcus AglB. The two distantly related oligosaccharyltransferases share unexpected structural similarity beyond that expected from the sequence comparison. The common architecture of the putative catalytic sites revealed a new catalytic motif in PglB. Site-directed mutagenesis analyses confirmed the contribution of this motif to the catalytic function. Bacterial PglB and archaeal AglB constitute a protein family of the catalytic subunit of OST along with STT3 from eukaryotes. A structure-aided multiple sequence alignment of the STT3/PglB/AglB protein family revealed three types of OST catalytic centers. This novel classification will provide a useful framework for understanding the enzymatic properties of the OST enzymes from Eukarya, Archaea, and Bacteria.

    Original languageEnglish
    Pages (from-to)4941-4950
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume285
    Issue number7
    DOIs
    Publication statusPublished - Feb 12 2010

    Fingerprint

    Archaea
    Eukaryota
    Catalytic Domain
    Bacteria
    Pyrococcus
    Glycosylation
    Mutagenesis
    Campylobacter jejuni
    Sequence Alignment
    Asparagine
    Site-Directed Mutagenesis
    Oligosaccharides
    Membrane Proteins
    Proteins
    Crystal structure
    dolichyl-diphosphooligosaccharide - protein glycotransferase
    Lipids
    Peptides
    Enzymes

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. / Maita, Nobuo; Nyirenda, James; Igura, Mayumi; Kamishikiryo, Jun; Kohda, Daisuke.

    In: Journal of Biological Chemistry, Vol. 285, No. 7, 12.02.2010, p. 4941-4950.

    Research output: Contribution to journalArticle

    Maita, Nobuo ; Nyirenda, James ; Igura, Mayumi ; Kamishikiryo, Jun ; Kohda, Daisuke. / Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 7. pp. 4941-4950.
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    AU - Kohda, Daisuke

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