Comparing spatial expression dynamics of bovine blastocyst under three different procedures: In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos

Hiroaki Nagatomo, Hiroki Akizawa, Ayari Sada, Yasunori Kishi, Ken Ichi Yamanaka, Tetsuya Takuma, Keisuke Sasaki, Nobuhiko Yamauchi, Yojiro Yanagawa, Masashi Nagano, Omohiro Kono, Masashi Takahashi, Manabu Kawahara

Research output: Contribution to journalArticle

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Abstract

There has been no work on spatiotemporal transcriptomic differences of blastocysts using in vivo- and in vitro-derived, and somatic cell nuclear transfer (SCNT) embryos. Here, effirst compared the lineage-differentially transcriptomic profiles of in vivo- and in vitroderived embryos by microarray analysis using divided into inner cell mass (ICM)-and trophectoderm (TE)-side samples, as well as those derived from SCNT in order to explore lineage-differentially expressed genes that are associated with preimplantation development in cattle. The transcriptomic profiles of the ICM-specific and TE-specific genes were similar between in vitro-derived embryos and in vivo-derived embryos, whereas SCNT embryos exhibited unusual lineage-differentially gene expression regulation at the blastocyst stage. The genes expressed in a spatiotemporal manner between developmentally normal in-vivo derived blastocysts and developmentally abnormal SCNT blastocysts might play critical roles for preimplantation development. Comparing spatial expression dynamics of bovine blastocyst under three different procedures revealed that CIITA was expressed in ICM-side samples of all the embryo types. CIITA is known as the master regulator of major histocompatibility complexes (MHC) class II genes that express in antigen-presenting cells but its biological function in preimplantation embryo is still unknown in mammals. Knockdown of CIITA expression in in vitro-derived embryos did not affect cleavage, but disrupted development of embryos into the blastocyst stage. These findings provide the novel transcriptomic information on blastocyst formation, raising the possibility that immune function-related gene directly plays important roles in bovine preimplantation development.

Original languageEnglish
Pages (from-to)159-171
Number of pages13
JournalJapanese Journal of Veterinary Research
Volume63
Issue number4
DOIs
Publication statusPublished - Jan 1 2015

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Embryo Transfer
Blastocyst
embryo transfer
somatic cells
blastocyst
embryo (animal)
transcriptomics
cattle
Embryonic Structures
genes
Genes
MHC Class II Genes
antigen-presenting cells
gene expression regulation
Gene Expression Regulation
major histocompatibility complex
Antigen-Presenting Cells
cells
Microarray Analysis
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Comparing spatial expression dynamics of bovine blastocyst under three different procedures : In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos. / Nagatomo, Hiroaki; Akizawa, Hiroki; Sada, Ayari; Kishi, Yasunori; Yamanaka, Ken Ichi; Takuma, Tetsuya; Sasaki, Keisuke; Yamauchi, Nobuhiko; Yanagawa, Yojiro; Nagano, Masashi; Kono, Omohiro; Takahashi, Masashi; Kawahara, Manabu.

In: Japanese Journal of Veterinary Research, Vol. 63, No. 4, 01.01.2015, p. 159-171.

Research output: Contribution to journalArticle

Nagatomo, H, Akizawa, H, Sada, A, Kishi, Y, Yamanaka, KI, Takuma, T, Sasaki, K, Yamauchi, N, Yanagawa, Y, Nagano, M, Kono, O, Takahashi, M & Kawahara, M 2015, 'Comparing spatial expression dynamics of bovine blastocyst under three different procedures: In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos', Japanese Journal of Veterinary Research, vol. 63, no. 4, pp. 159-171. https://doi.org/10.14943/jjvr.63.4.159
Nagatomo, Hiroaki ; Akizawa, Hiroki ; Sada, Ayari ; Kishi, Yasunori ; Yamanaka, Ken Ichi ; Takuma, Tetsuya ; Sasaki, Keisuke ; Yamauchi, Nobuhiko ; Yanagawa, Yojiro ; Nagano, Masashi ; Kono, Omohiro ; Takahashi, Masashi ; Kawahara, Manabu. / Comparing spatial expression dynamics of bovine blastocyst under three different procedures : In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos. In: Japanese Journal of Veterinary Research. 2015 ; Vol. 63, No. 4. pp. 159-171.
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