Abstract
There has been no work on spatiotemporal transcriptomic differences of blastocysts using in vivo- and in vitro-derived, and somatic cell nuclear transfer (SCNT) embryos. Here, effirst compared the lineage-differentially transcriptomic profiles of in vivo- and in vitroderived embryos by microarray analysis using divided into inner cell mass (ICM)-and trophectoderm (TE)-side samples, as well as those derived from SCNT in order to explore lineage-differentially expressed genes that are associated with preimplantation development in cattle. The transcriptomic profiles of the ICM-specific and TE-specific genes were similar between in vitro-derived embryos and in vivo-derived embryos, whereas SCNT embryos exhibited unusual lineage-differentially gene expression regulation at the blastocyst stage. The genes expressed in a spatiotemporal manner between developmentally normal in-vivo derived blastocysts and developmentally abnormal SCNT blastocysts might play critical roles for preimplantation development. Comparing spatial expression dynamics of bovine blastocyst under three different procedures revealed that CIITA was expressed in ICM-side samples of all the embryo types. CIITA is known as the master regulator of major histocompatibility complexes (MHC) class II genes that express in antigen-presenting cells but its biological function in preimplantation embryo is still unknown in mammals. Knockdown of CIITA expression in in vitro-derived embryos did not affect cleavage, but disrupted development of embryos into the blastocyst stage. These findings provide the novel transcriptomic information on blastocyst formation, raising the possibility that immune function-related gene directly plays important roles in bovine preimplantation development.
| Original language | English |
|---|---|
| Pages (from-to) | 159-171 |
| Number of pages | 13 |
| Journal | Japanese Journal of Veterinary Research |
| Volume | 63 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 2015 |
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All Science Journal Classification (ASJC) codes
- veterinary(all)
Cite this
Comparing spatial expression dynamics of bovine blastocyst under three different procedures : In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos. / Nagatomo, Hiroaki; Akizawa, Hiroki; Sada, Ayari; Kishi, Yasunori; Yamanaka, Ken Ichi; Takuma, Tetsuya; Sasaki, Keisuke; Yamauchi, Nobuhiko; Yanagawa, Yojiro; Nagano, Masashi; Kono, Omohiro; Takahashi, Masashi; Kawahara, Manabu.
In: Japanese Journal of Veterinary Research, Vol. 63, No. 4, 01.01.2015, p. 159-171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparing spatial expression dynamics of bovine blastocyst under three different procedures
T2 - In-vivo, in-vitro derived, and somatic cell nuclear transfer embryos
AU - Nagatomo, Hiroaki
AU - Akizawa, Hiroki
AU - Sada, Ayari
AU - Kishi, Yasunori
AU - Yamanaka, Ken Ichi
AU - Takuma, Tetsuya
AU - Sasaki, Keisuke
AU - Yamauchi, Nobuhiko
AU - Yanagawa, Yojiro
AU - Nagano, Masashi
AU - Kono, Omohiro
AU - Takahashi, Masashi
AU - Kawahara, Manabu
PY - 2015/1/1
Y1 - 2015/1/1
N2 - There has been no work on spatiotemporal transcriptomic differences of blastocysts using in vivo- and in vitro-derived, and somatic cell nuclear transfer (SCNT) embryos. Here, effirst compared the lineage-differentially transcriptomic profiles of in vivo- and in vitroderived embryos by microarray analysis using divided into inner cell mass (ICM)-and trophectoderm (TE)-side samples, as well as those derived from SCNT in order to explore lineage-differentially expressed genes that are associated with preimplantation development in cattle. The transcriptomic profiles of the ICM-specific and TE-specific genes were similar between in vitro-derived embryos and in vivo-derived embryos, whereas SCNT embryos exhibited unusual lineage-differentially gene expression regulation at the blastocyst stage. The genes expressed in a spatiotemporal manner between developmentally normal in-vivo derived blastocysts and developmentally abnormal SCNT blastocysts might play critical roles for preimplantation development. Comparing spatial expression dynamics of bovine blastocyst under three different procedures revealed that CIITA was expressed in ICM-side samples of all the embryo types. CIITA is known as the master regulator of major histocompatibility complexes (MHC) class II genes that express in antigen-presenting cells but its biological function in preimplantation embryo is still unknown in mammals. Knockdown of CIITA expression in in vitro-derived embryos did not affect cleavage, but disrupted development of embryos into the blastocyst stage. These findings provide the novel transcriptomic information on blastocyst formation, raising the possibility that immune function-related gene directly plays important roles in bovine preimplantation development.
AB - There has been no work on spatiotemporal transcriptomic differences of blastocysts using in vivo- and in vitro-derived, and somatic cell nuclear transfer (SCNT) embryos. Here, effirst compared the lineage-differentially transcriptomic profiles of in vivo- and in vitroderived embryos by microarray analysis using divided into inner cell mass (ICM)-and trophectoderm (TE)-side samples, as well as those derived from SCNT in order to explore lineage-differentially expressed genes that are associated with preimplantation development in cattle. The transcriptomic profiles of the ICM-specific and TE-specific genes were similar between in vitro-derived embryos and in vivo-derived embryos, whereas SCNT embryos exhibited unusual lineage-differentially gene expression regulation at the blastocyst stage. The genes expressed in a spatiotemporal manner between developmentally normal in-vivo derived blastocysts and developmentally abnormal SCNT blastocysts might play critical roles for preimplantation development. Comparing spatial expression dynamics of bovine blastocyst under three different procedures revealed that CIITA was expressed in ICM-side samples of all the embryo types. CIITA is known as the master regulator of major histocompatibility complexes (MHC) class II genes that express in antigen-presenting cells but its biological function in preimplantation embryo is still unknown in mammals. Knockdown of CIITA expression in in vitro-derived embryos did not affect cleavage, but disrupted development of embryos into the blastocyst stage. These findings provide the novel transcriptomic information on blastocyst formation, raising the possibility that immune function-related gene directly plays important roles in bovine preimplantation development.
UR - http://www.scopus.com/inward/record.url?scp=84949658403&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949658403&partnerID=8YFLogxK
U2 - 10.14943/jjvr.63.4.159
DO - 10.14943/jjvr.63.4.159
M3 - Article
C2 - 26753242
AN - SCOPUS:84949658403
VL - 63
SP - 159
EP - 171
JO - Japanese Journal of Veterinary Research
JF - Japanese Journal of Veterinary Research
SN - 0047-1917
IS - 4
ER -