Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes

Ryo Asato, Shigeo Yoshida, Atsushi Ogura, Takahito Nakama, Keijiro Ishikawa, shintaro nakao, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Kazuho Ikeo, Takashi Gojobori, Toshihiro Kono, Tatsuro Ishibashi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Proliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of epiretinal membranes (ERMs) associated with a PVR (PVR-ERM) and to compare it to the expression profile of less-aggressive secondary ERMs. Methodology/Principal Findings: A PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from ERMs obtained during vitrectomy. The sequence from the 5′ end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). We obtained 1116 nonredundant clusters representing individual genes expressed in PVR-ERMs, and 799 clusters representing the genes expressed in secondary ERMs. The transcriptome of the PVR-ERMs was subdivided by functional subsets of genes related to metabolism, cell adhesion, cytoskeleton, signaling, and other functions, by FatiGo analysis. The genes highly expressed in PVR-ERMs were compared to those expressed in the secondary ERMs, and these were subdivided by cell adhesion, proliferation, and other functions. Querying 10 cell adhesion-related genes against the STRING database yielded 70 possible physical relationships to other genes/proteins, which included an additional 60 genes that were not detected in the PVR-ERM library. Of these, soluble CD44 and soluble vascular cellular adhesion molecule-1 were significantly increased in the vitreous of patients with PVR. Conclusions/Significance: Our results support an earlier hypothesis that a PVR-ERM, even from genomic points of view, is an aberrant form of wound healing response. Genes preferentially expressed in PVR-ERMs may play an important role in the progression of PVR and could be served as therapeutic targets.

Original languageEnglish
Article numbere54191
JournalPloS one
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 29 2013

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Epiretinal Membrane
Proliferative Vitreoretinopathy
Transcriptome
Gene expression
eyes
Membranes
gene expression
cell adhesion
Genes
genes
Cell adhesion
Cell Adhesion
Retinal Detachment
cytoskeleton
tissue repair
expressed sequence tags
multigene family
blood vessels
transcriptome
cDNA libraries

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes. / Asato, Ryo; Yoshida, Shigeo; Ogura, Atsushi; Nakama, Takahito; Ishikawa, Keijiro; nakao, shintaro; Sassa, Yukio; Enaida, Hiroshi; Oshima, Yuji; Ikeo, Kazuho; Gojobori, Takashi; Kono, Toshihiro; Ishibashi, Tatsuro.

In: PloS one, Vol. 8, No. 1, e54191, 29.01.2013.

Research output: Contribution to journalArticle

Asato, Ryo ; Yoshida, Shigeo ; Ogura, Atsushi ; Nakama, Takahito ; Ishikawa, Keijiro ; nakao, shintaro ; Sassa, Yukio ; Enaida, Hiroshi ; Oshima, Yuji ; Ikeo, Kazuho ; Gojobori, Takashi ; Kono, Toshihiro ; Ishibashi, Tatsuro. / Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes. In: PloS one. 2013 ; Vol. 8, No. 1.
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AU - Ishikawa, Keijiro

AU - nakao, shintaro

AU - Sassa, Yukio

AU - Enaida, Hiroshi

AU - Oshima, Yuji

AU - Ikeo, Kazuho

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