Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data

M. M. Ribeiro, S. Mariette, G. G. Vendramin, A. E. Szmidt, C. Plomion, A. Kremer

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Abstract

We compared the genetic variation of Pinus pinaster populations using amplified fragment length polymorphism (AFLP) and chloroplast simple-sequence repeat (cpSSR) loci. Populations' levels of diversity within groups were found to be similar with AFLPs, but not with cpSSRs. The high interlocus variance associated with the AFLP loci could account for the lack of differences in the former. Although AFLPs revealed much lower genetic diversity than cpSSRs, the levels of among-population differentiation found with the two types of marker were similar, provided that loci showing fewer than four null-homozygotes, in any population, were pruned from the AFLP data. Moreover, the French and Portuguese populations were clearly differentiated from each other, with both markers. The Mantel test showed that the genetic distance matrix calculated using the AFLP data was correlated with the matrix derived from the cpSSRs. Because of the concordance found between markers we conclude that gene flow was indeed the predominant force shaping nuclear and chloroplastic genetic variation of the populations within regions, at the geographical scale studied.

Original languageEnglish
Pages (from-to)869-877
Number of pages9
JournalMolecular Ecology
Volume11
Issue number5
DOIs
Publication statusPublished - May 27 2002

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Pinus
Pinus pinaster
Chloroplasts
chloroplast
Microsatellite Repeats
amplified fragment length polymorphism
polymorphism
chloroplasts
microsatellite repeats
genetic variation
Population
loci
matrix
gene flow
Gene Flow
Homozygote
homozygosity
genetic distance
genetic diversity
comparison

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data. / Ribeiro, M. M.; Mariette, S.; Vendramin, G. G.; Szmidt, A. E.; Plomion, C.; Kremer, A.

In: Molecular Ecology, Vol. 11, No. 5, 27.05.2002, p. 869-877.

Research output: Contribution to journalArticle

Ribeiro, M. M. ; Mariette, S. ; Vendramin, G. G. ; Szmidt, A. E. ; Plomion, C. ; Kremer, A. / Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data. In: Molecular Ecology. 2002 ; Vol. 11, No. 5. pp. 869-877.
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