Comparison of histological responses and tissue damage expansion between resistant and susceptible Pinus thunbergii infected with pine wood nematode Bursaphelenchus xylophilus

Dai Kusumoto, Takashi Yonemichi, Hiroki Inoue, Tomonori Hirao, Atsushi Watanabe, Toshihiro Yamada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Pine wilt disease caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, has been epidemic and has had disastrous impacts on pine forests and forest ecosystems in eastern Asia. Many pine species in this area are susceptible to this disease. Pinus thunbergii is particularly susceptible. In Japan, tree breeders have selected surviving trees from severely damaged forests as resistant candidates, and have finally established several resistant varieties of P. thunbergii. However, this breeding procedure requires much time and effort due to the lack of physiological and phenotypical information about resistance. To investigate the resistance mechanisms of selected P. thunbergii, we compared histochemical responses, tissue damage expansion, and PWN distribution in resistant and susceptible clones of P. thunbergii after PWN inoculation. The results suggested that the mechanisms of resistance are as follows: damage expansion in the cortex, cambium, and xylem axial resin canals are retarded in resistant trees soon after inoculation, probably due to the induction of wall protein-based defenses. Suppression of PWN reproduction was particularly caused by inhibition of damage expansion in the cambium. The slow expansion of damage in each tissue provides time for the host to complete the biosynthesis of lignin in the walls of cells that surround the damaged regions. This lignification of cell walls is assumed to effectively inhibit the migration and reproduction of the PWNs. The mechanism of initial damage retardation is presumed to be a key for resistance.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalJournal of Forest Research
Volume19
Issue number2
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Pinus thunbergii
Bursaphelenchus xylophilus
nematode
damage
cambium
resistance mechanisms
inoculation
Pinus
cell walls
resin canals
wilt
lignification
biological resistance
xylem
East Asia
forest ecosystems
forest ecosystem
lignin
coniferous forests
canal

All Science Journal Classification (ASJC) codes

  • Forestry

Cite this

Comparison of histological responses and tissue damage expansion between resistant and susceptible Pinus thunbergii infected with pine wood nematode Bursaphelenchus xylophilus. / Kusumoto, Dai; Yonemichi, Takashi; Inoue, Hiroki; Hirao, Tomonori; Watanabe, Atsushi; Yamada, Toshihiro.

In: Journal of Forest Research, Vol. 19, No. 2, 04.2014, p. 285-294.

Research output: Contribution to journalArticle

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