Comparison of pyrimidine nucleotide synthetic enzymes involved in 5‐fluorouracil metabolism between human adenocarcinomas and squamous cell carcinomas

Yoshihiko Maehara, Sunao Moriguchi, Yasunori Emi, Akihiro Watanabe, Shunji Kohnoe, Shunichi Tsujitani, Keizo Sugimachi

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The activities of orotate phosphoribosyltransferase (OPRT), cytidine triphosphate (CTP) synthetase, deoxycytidine monophosphate (dCMP) deaminase, thymidine monophosphate (dTMP) kinase, uridine (Urd) kinase, thymidine (dThd) kinase, Urd and dThd phosphorylases, and DNA polymerase were examined in the eight human lung squamous cell carcinomas and five lung adenocarcinomas, and five tumor‐adjacent normal lung tissues. All of these enzymes are involved in pyrimidine nucleotide synthesis. The metabolism of 5‐fluorouracil (5‐FU) was determined. The levels of these enzymes, except for OPRT, were high in tumor tissues and almost the same between lung squamous cell carcinomas and adenocarcinomas, with no statistical difference. The activities for phosphorylation and degradation of 5‐FU were similar in each tissue type of tumor. As 5‐FU is incorporated into tumor cells and is metabolized actively to 5‐FU nucleotides in squamous cell carcinoma tissues, at almost the same level seen in adenocarcinoma tissues, this drug should have a wide clinical application.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalCancer
Volume66
Issue number1
DOIs
Publication statusPublished - Jul 1 1990

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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