Comparison of retention behavior between supercritical fluid chromatography and normal-phase high-performance liquid chromatography with various stationary phases

Tsunehisa Hirose, Daniel Keck, Yoshihiro Izumi, Takeshi Bamba

Research output: Contribution to journalArticle

Abstract

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and p-p interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

Original languageEnglish
Article number2425
JournalMolecules
Volume24
Issue number13
DOIs
Publication statusPublished - Jan 1 2019

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Supercritical Fluid Chromatography
supercritical fluids
liquid chromatography
High performance liquid chromatography
chromatography
High Pressure Liquid Chromatography
selectivity
Silica Gel
silica gel
Hydrophobic and Hydrophilic Interactions
Adsorption
adsorption
interactions

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

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