Comparison of the antiviral potentials among the pseudorabies-resistant transgenes encoding different soluble forms of porcine nectin-1 in transgenic mice

Etsuro Ono, Yukiko Tomioka, Yuki Watanabe, Keiko Amagai, Masami Morimatsu, Kyoko Shinya, Pierre Cherel

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D by the first immunoglobulin (lg)-like domain. The possibility of making animals resistant to pseudorabies virus (PRV) infection has been investigated by generating transgenic mice expressing soluble forms of porcine nectin-1. Previously, transgenic mice were generated that expressed a fusion protein made of the entire ectodomain of nectin-1 fused to the Fc portion of human IgG, or the first Ig-like domain fused to the Fc portion of porcine IgG. Here, the contribution of the second and third Ig-like domains of nectin-1 was analysed by generating transgenic mice expressing the entire ectodomain of nectin-1 fused to the porcine Fc portion. Transgenic mice expressing each of three different fusion proteins were challenged with PRV for comparison of their resistance. Altogether, mice transgenic for a chimera that carried the entire ectodomain were more resistant than those transgenic for a chimera that carried the first Ig-like domain.

Original languageEnglish
Pages (from-to)2636-2641
Number of pages6
JournalJournal of General Virology
Volume88
Issue number10
DOIs
Publication statusPublished - Oct 2007

All Science Journal Classification (ASJC) codes

  • Virology

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