Comparison of the bactericidal effect of slightly acidic hypochlorous water with that of conventional sterilizers

Kevin Webby Soli, Ai Motomatsu, Asako Yoshizumi, Mami Yamakawa, Tomoko Mishima, Ken-ichi Honjoh, Takahisa Miyamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The bactericidal effect of slightly acidic hypochlorous water (SAHW) on Salmonella Enteritidis, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus, as well as some bacterial strains isolated from fresh lettuce was evaluated. Viable counts of all tested bacterial samples decreased immediately after treatment by SAHW. Most bacterial cells with the exception of B. cereus, and S. aureus were not culturable on TSA after treatment by 1 to 30 mg/L SAHW. Likewise, Pseudomonas sp., and Flavobacterium or Xanthomonas sp., Kurthia sp., Micrococcus sp., and Corynebacterium or Microbacterium sp. were not culturable on TSA after treatment by 30 mg/L SAHW. Viable counts of S. aureus, E. coli, Flavobacterium or Xanthomonas sp., and Pseudomonas sp. showed a 5 to 6 log cfu/mL reduction at day 0 and maintained a count of less than 1 log cfu/mL from day 1 to day 7 following treatment by 30 mg/L SAHW. Sodium hypochlorite (NaOCl, 0.5-1.0 mg/L) decreased the viable counts of S. Enteritidis to less than the lower limit of detection, 1 log cfu/mL, from day 1 to day 7 following treatment by 1 mg/L. NaOCl was not sufficient at 0.5-0.75 mg/L in reducing viable counts of S. Enteritidis because of a 2 to 5 log cfu/mL increase from day 2 to day 5 due to recovery from injury. Initial counts of S. Enteritidis after hydrogen peroxide (H2O2, 1000-2000 mg/L) treatment slowly decreased over time to less than 1 log cfu/mL after day 2. Treatment by 1750 to 2000 mg/L H2O2 has sufficient bactericidal activity on S. Enteritidis cells, however, at a higher concentration compared to NaOCl or SAHW SAHW decreased viability of S. Enteritidis immediately with higher reduction counts in 1,5, and 30 mg/L from day 0 to day 7 unlike NaOCl and H2O2.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume55
Issue number2
Publication statusPublished - Oct 2010

Fingerprint

sterilizers
antibacterial properties
Salmonella enteritidis
Salmonella Enteritidis
Water
Xanthomonas
Flavobacterium
water
Staphylococcus aureus
Bacillus cereus
Pseudomonas
Kurthia
Microbacterium
Escherichia coli
Sodium Hypochlorite
Micrococcus
Lettuce
Corynebacterium
sodium hypochlorite
Listeria monocytogenes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Biotechnology

Cite this

Comparison of the bactericidal effect of slightly acidic hypochlorous water with that of conventional sterilizers. / Soli, Kevin Webby; Motomatsu, Ai; Yoshizumi, Asako; Yamakawa, Mami; Mishima, Tomoko; Honjoh, Ken-ichi; Miyamoto, Takahisa.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 55, No. 2, 10.2010, p. 275-280.

Research output: Contribution to journalArticle

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