The comparison between the compaction of single chains and bundling of multiple chains in giant DNA molecules was studied using the fluorescence microscopy. It was found that when the concentration of DNA was below 1 μM in base-pair units, individual DNA molecules exhibit a transition from an elongated state to a compact state. It was observed that when the concentration of DNA was increased to 10 μM, a thick fiberlike assembly of multiple chains appears. The transition between single-chain compaction and bundle formation with multiple-chain assemblies was reproduced by a theoretical calculation.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry