Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules

Toshio Iwataki; Satoru Kidoaki; Takahiro Sakaue; Kenichi Yoshikawa; Sergey S. Abramchuk

doi:10.1063/1.1642610

Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules

Toshio Iwataki, Satoru Kidoaki, Takahiro Sakaue, Kenichi Yoshikawa, Sergey S. Abramchuk

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

The comparison between the compaction of single chains and bundling of multiple chains in giant DNA molecules was studied using the fluorescence microscopy. It was found that when the concentration of DNA was below 1 μM in base-pair units, individual DNA molecules exhibit a transition from an elongated state to a compact state. It was observed that when the concentration of DNA was increased to 10 μM, a thick fiberlike assembly of multiple chains appears. The transition between single-chain compaction and bundle formation with multiple-chain assemblies was reproduced by a theoretical calculation.

Original language	English
Pages (from-to)	4004-4011
Number of pages	8
Journal	Journal of Chemical Physics
Volume	120
Issue number	8
DOIs	https://doi.org/10.1063/1.1642610
Publication status	Published - Feb 22 2004

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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10.1063/1.1642610

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AU - Abramchuk, Sergey S.

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Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules

Abstract

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