Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules

Toshio Iwataki, Satoru Kidoaki, Takahiro Sakaue, Kenichi Yoshikawa, Sergey S. Abramchuk

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The comparison between the compaction of single chains and bundling of multiple chains in giant DNA molecules was studied using the fluorescence microscopy. It was found that when the concentration of DNA was below 1 μM in base-pair units, individual DNA molecules exhibit a transition from an elongated state to a compact state. It was observed that when the concentration of DNA was increased to 10 μM, a thick fiberlike assembly of multiple chains appears. The transition between single-chain compaction and bundle formation with multiple-chain assemblies was reproduced by a theoretical calculation.

Original languageEnglish
Pages (from-to)4004-4011
Number of pages8
JournalJournal of Chemical Physics
Volume120
Issue number8
DOIs
Publication statusPublished - Feb 22 2004

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Compaction
deoxyribonucleic acid
Molecules
DNA
molecules
Fluorescence microscopy
assemblies
bundles
assembly
microscopy
fluorescence

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules. / Iwataki, Toshio; Kidoaki, Satoru; Sakaue, Takahiro; Yoshikawa, Kenichi; Abramchuk, Sergey S.

In: Journal of Chemical Physics, Vol. 120, No. 8, 22.02.2004, p. 4004-4011.

Research output: Contribution to journalArticle

Iwataki, Toshio ; Kidoaki, Satoru ; Sakaue, Takahiro ; Yoshikawa, Kenichi ; Abramchuk, Sergey S. / Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules. In: Journal of Chemical Physics. 2004 ; Vol. 120, No. 8. pp. 4004-4011.
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